Exploration of serum sensitive biomarkers of fatty liver in dairy cows

Abstract

Serum proteins are sensitive with diseases in dairy cows, and some of them could be used as biomarkers for fatty liver. This study aimed to explore serum biomarkers for fatty liver in dairy cows. A total of 28 early lactating dairy cows were chosen from a commercial dairy herds, liver samples were collected for determining concentration of triacylglycerol (TAG), and serum samples were collected for measuring fibroblast growth factor-21 (FGF-21), adiponectin, Lipoprotein-associated phospholipase A2 (LP-PLA2), and hemoglobin (Hb). Dairy cows were divided into fatty liver (liver TAG > 5%, wet weight) and control group (liver TAG < 5%, wet weight). Concentration of FGF-21 was greater in fatty liver cows, while the concentration of LP-PLA2 and Hb was less. The concentration of FGF-21 and total Hb had strong correlation with the liver TAG as well as good prediction power (kappa value = 0.79 and 0.58, respectively). These results suggested that the serum concentration of FGF-21 and total Hb could be potentially used as fatty liver biomarkers in lactating dairy cows.

Figure 1

The relationship between serum fibroblast growth factor-21 (FGF-21) and liver triglyceride concentration (r = 0.74; P < 0.01).

Figure 2

The relationship between serum hemoglobin and liver triglyceride concentration (r = −0.65; P < 0.01).

Figure 3

Receiver operating characteristic (ROC) curve of fibroblast growth factor-21 (FGF-21) with 95% confidence intervals. The area under curve (AUC) is 0.946 (from 0.872 to 1), and the cut-off value is 1015 pg/mL with the sensitivity of 86.7% and specificity of 92.3%.

Figure 4

Receiver operating characteristic (ROC) curve of hemoglobin with 95% confidence intervals. The area under curve (AUC) is 0.821 (from 0.660 to 0.981), and the cut-off value is 41.5 μg/mL with the sensitivity of 92.3% and specificity of 66.7%.