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. 2018 Aug 31:5:905-909.
doi: 10.1016/j.toxrep.2018.08.020. eCollection 2018.

SDS-PAGE technique as biomarker for fish toxicological studies

Ola I Muhammad  1 , Usama M Mahmoud  1 , Francesco Fazio  2 , Alaa El-Din H Sayed  1
Affiliations

SDS-PAGE technique as biomarker for fish toxicological studies

Ola I Muhammad et al. Toxicol Rep. .

Erratum in

Abstract

Although many studies on the hematological and biochemical parameters in fishes have been done, still there are some shortage in the estimation and evaluation of the baseline's values of marine and freshwater fishes. Recently, the use of hematology and biochemistry of fishes in toxicology, aquaculture, environmental pollution, feeding, and antioxidants studies has been increased. In this study we introduced the importance of those parameters and their importance as biomarkers in fish toxicology from previous literature and as new findings. Hemato-biochemical parameters were widely used in fish toxicological studies. Many researches have used the protein electrophoresis as a valid tool to determining intra and inter-specific variation among species. Protein profile was extensively used in determining the health of fish, as indicators of anemia or other diseases provide information about the existence of the disease, and in the diagnosis of disease. So, to carry out the aim of this study, we reported one of the more advanced techniques used SDS-PAGE as molecular biomarker for protein profile analysis in fish with shedding the light on the importance of hematological and biochemical parameters in fish toxicological studies.

Keywords: Biochemistry; Fish; Hematology; Liver; SDS-PAGE.

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Figures

None
Graphical abstract
Fig. 1
Fig. 1
Photographs of the studied fishes (a) Diplodus noct [48], (b) Parupeneus forsskali, (c) Thalassoma klunzingeri.
Fig. 2
Fig. 2
Electropherogram of Thalassoma klunzingeri (lanes 1a, 1b & 1c), Diplodus noct (lanes 2a–2c) and Parupeneus forsskali (lanes 3a, 3b & 3c) by using liver protein by SDS-PAGE. Densitometric analysis of lane 1a (a), lane 2b (b) and lane 3a (c).
Fig. 3
Fig. 3
Cluster analysis for three Red Sea fishes based on profile.
See this image and copyright information in PMC

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