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. 2018 Oct;7(10):705-708.
doi: 10.1002/sctm.18-0017. Epub 2018 Sep 13.

Long-Term Engraftment (16 Years) of Myoblasts in a Human Infarcted Heart

Marie Crahès  1   2 , Marie-Cécile Bories  3 , Jean-Thomas Vilquin  4 , Jean-Pierre Marolleau  5 , Michel Desnos  2   6 , Jérôme Larghero  7   8   9 , Gilles Soulat  2   10 , Patrick Bruneval  1   2   6 , Albert A Hagège  2   6   11 , Philippe Menasché  2   3   6
Affiliations

Long-Term Engraftment (16 Years) of Myoblasts in a Human Infarcted Heart

Marie Crahès et al. Stem Cells Transl Med. 2018 Oct.

Abstract

We report the case of a patient who had undergone injections of myoblasts in an infarct area 16 years before being referred for heart transplantation. The pathological examination of the explanted heart found persisting myotubes embedded in fibrosis. This finding supports the ability of myoblasts to survive in harsh environments, which can make them appealing candidates for transplantation in diseases requiring supply of new myogenic cells. Stem Cells Translational Medicine 2018;7:705-708.

Keywords: Clinical cell transplantation; Heart failure; Long-term follow-up; Myoblasts.

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Figures

Figure 1
Figure 1
Histological and immunochemical findings in the native heart at the site of the cell‐transplanted myocardial infarction. (A): Multinucleated skeletal muscle cells/myotubes (arrows) embedded in fibrosis. Inset shows the Z‐bands in a skeletal muscle cell. Hematoxylin & Eosin staining. Original ×40 and ×100 for inset. (B): Large sub‐epicardial focus (black outline) of grafted skeletal muscle cells expressing the fast isoform of the skeletal muscle myosin heavy chain. Original ×2.5. (C): Strong expression of the fast isoform of the skeletal muscle myosin heavy chain (MYH1) by several cells. Although the density of skeletal muscle cells is high, they are separated by fibrosis. Original ×20. (D): Expression of the slow isoform of the skeletal muscle myosin heavy chain (MYH7) by a small proportion of the grafted cells. Original ×20. (E): Expression of the skeletal troponin T (TNNT3) fast isoform by the grafted skeletal muscle cells. Original ×40. (F): Expression of the myogenin transcription factor in nuclei of the grafted skeletal muscle cells (arrow). Original ×40. (G): Expression of CD56 by myotubes. Original ×40.
See this image and copyright information in PMC

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