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. 1977 Jul 20;35(1):57-64.
doi: 10.1007/BF01006965.

PHA and endotoxin stimulation of human lymphocytes separated by albumin density gradient centrifugation

A Biermann, D G von Keyserlingk, I Boll

PHA and endotoxin stimulation of human lymphocytes separated by albumin density gradient centrifugation

A Biermann et al. Blut. .

Abstract

Venous blood from eight healthy subjects was divided into four fractions on a discontinuous albumin density gradient. The percentage recovery of lymphocytes was 82.3%; the purity of the lymphocyte fractions was 83.6%. The lymphocytes were cultured with PHA and Endotoxin, and the samples were analysed after 24, 48, and 72 hours. After PHA stimulation immunoblasts appeared up to 59.3% in the cultures from the 19-21% albumin fraction. After Endotoxin stimulation the maximum (75.8%) was reached in the heavy (25-27% albumin) fraction. Thus, it is concluded that the lymphocytes which can be stimulated with both the mitogens have different densities, the PHA-stimulable T lymphocytes being ligther than the Endotoxin-stimulable B lymphocytes. It is also concluded that as a mitogen Endotoxin is equal to PHA.

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