Structure of a human retroviral sequence related to mouse mammary tumor virus

Abstract

Human sequences that are related to the mouse mammary tumor virus (MuMTV) genome were cloned from breast tumor cell DNA. Of 100 recombinants, only 1 hybridized with two different probes from separate regions of the MuMTV genome (gag-pol and long terminal repeat [LTR]). This sequence, NMWV 4, was shown to have a proviruslike structure. Hybridization to digests of normal and tumor cell DNA indicated that NMWV 4 and a few closely related sequences are endogenous to the human genome. The regions that contain homology to the MuMTV LTR were sequenced. Long repeated sequences with the hallmarks of retroviral LTRs were identified. The NMWV 4 LTR contains transcription initiation and termination signals and is flanked by a polypurine tract (5' LTR) and a primer-binding site (3' LTR). The primer-binding site is complementary to tRNA lysine, the primer used by MuMTV and HTLV-III. The polypurine tract is also similar to those of these two retroviruses.