Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes

Mathilde Lescat¹, Laurent Poirel², Patrice Nordmann³

Affiliations

¹ Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA, IAME), Paris, France; Université Paris Nord, Sorbonne Paris Cité, Paris, France; AP-HP, Hôpitaux Universitaires Paris Seine Saint-Denis, Bondy, France.
² Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA, IAME), Paris, France; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland. Electronic address: laurent.poirel@unifr.ch.
³ Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA, IAME), Paris, France; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland; University of Lausanne and University Hospital Center, Lausanne, Switzerland.

PMID: 30220493
DOI: 10.1016/j.diagmicrobio.2018.04.010

Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes

Mathilde Lescat et al. Diagn Microbiol Infect Dis. 2018 Dec.

. 2018 Dec;92(4):267-269.

doi: 10.1016/j.diagmicrobio.2018.04.010. Epub 2018 Apr 22.

Authors

Mathilde Lescat¹, Laurent Poirel², Patrice Nordmann³

Affiliations

¹ Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA, IAME), Paris, France; Université Paris Nord, Sorbonne Paris Cité, Paris, France; AP-HP, Hôpitaux Universitaires Paris Seine Saint-Denis, Bondy, France.
² Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA, IAME), Paris, France; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland. Electronic address: laurent.poirel@unifr.ch.
³ Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA, IAME), Paris, France; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland; University of Lausanne and University Hospital Center, Lausanne, Switzerland.

PMID: 30220493
DOI: 10.1016/j.diagmicrobio.2018.04.010

Abstract

A rapid (total time <2 h) and reliable multiplex polymerase chain reaction for screening of mcr-1 to mcr-5 genes conferring resistance to colistin has been developed. This technique has been tested on a collection of isolates previously identified as bearing mcr-1, mcr-2, and mcr-like genes and had a sensitivity and a specificity of 100%. Using this method, we were also able to identify a single isolate possessing both mcr-1 and mcr-5 genes.

Keywords: Multiplex PCR; Polymyxin resistance6; mcr genes.

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Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes

Affiliations

Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes

Authors

Affiliations

Abstract

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LinkOut - more resources

Full Text Sources