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. 2018 Jul 10:19:2047-2052.
doi: 10.1016/j.dib.2018.06.089. eCollection 2018 Aug.

HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification

Anna Grebinyk  1   2   3 , Sergii Grebinyk  1 , Svitlana Prylutska  4 , Uwe Ritter  5 , Olga Matyshevska  3 , Thomas Dandekar  2 , Marcus Frohme  1
Affiliations

HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification

Anna Grebinyk et al. Data Brief. .

Abstract

The presented dataset describes the quantification of carbon nanoparticle C60 fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography-electro spray ionization-mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C60 fullerene. Then, human leukemic cells were incubated with C60 fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction. The C60 fullerene content was quantified by HPLC-ESI-MS in extracts of cellular fractions. This data article refers to the research article "C60 Fullerene Accumulation in Human Leukemic Cells and Perspectives of LED-mediated Photodynamic Therapy" by Grebinyk et al. [1].

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Figures

Fig. 1
Fig. 1
Data of the developed HPLC-ESI-MS method for C60 fullerene detection and quantification: A – representative SIM-chromatogram of C60 fullerene from cell extract, B – calibration curve with methods performance characteristics used for nanoparticle content quantification.
Fig. 2
Fig. 2
C60 fullerene mitochondrial content quantification: A – Isolation of mitochondria, B – Succinate-reductase activity of obtained cellular fractions, C – C60 fullerene distribution among cellular fractions.
See this image and copyright information in PMC

References

    1. A. Grebinyk, S. Grebinyk, S. Prylutska, U. Ritter, O. Matyshevska, T. Dandekar and M. Frohme, C60 fullerene accumulation in human leukemic cells and perspectives of LED-mediated photodynamic therapy. Free Radic. Biol. Med. 27, 2018, 124:319-327, 10.1016/j.freeradbiomed.2018.06.022. - DOI - PubMed
    1. Ritter U., Prylutskyy Y.I., Evstigneev M.P., Davidenko N.A., Cherepanov V.V., Senenko A.I., Marchenko O.A., Naumovets A.G. Structural features of highly stable reproducible C60 Fullerene aqueous colloid solution probed by various techniques. Fuller. Nanotub. Carbon Nanostruct. 2015;23:530–534.
    1. Frezza C., Cipolat S., Scorrano L. Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts. Nat. Protoc. 2007;2(2):287–295. - PubMed
    1. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976;72:248–254. - PubMed
    1. Pennington R.J. Mitochondrial succinate-tetrazoliumreductase and adenosine triphosphatase. J. Biochem. 1961;80:649–654. (PMCID:PMC1243280) - PMC - PubMed

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