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. 2018 Sep 4:9:1870.
doi: 10.3389/fmicb.2018.01870. eCollection 2018.

The Influence of Microwave Sterilization on the Ultrastructure, Permeability of Cell Membrane and Expression of Proteins of Bacillus Cereus

Jin-Xuan Cao  1 , Fang Wang  1 , Xuan Li  1 , Yang-Ying Sun  1 , Ying Wang  1 , Chang-Rong Ou  1 , Xing-Feng Shao  1 , Dao-Dong Pan  1 , Dao-Ying Wang  2
Affiliations

The Influence of Microwave Sterilization on the Ultrastructure, Permeability of Cell Membrane and Expression of Proteins of Bacillus Cereus

Jin-Xuan Cao et al. Front Microbiol. .

Abstract

Bacillus cereus was isolated from ready-to-serve brine goose, identified by 16S rRNA gene sequencing analysis and treated with a commercial microwave sterilization condition (a power of 1,800 W at 85°C for 5 min). The influence of microwaves on the morphology, the permeability of membrane and the expression of total bacterial proteins was observed. Microwave induced the clean of bacterial nuclear chromatin, increased the permeability and disrupted the integrity of membrane. Twenty-three proteins including 18 expressed down-regulated proteins and 5 expressed up-regulated proteins were identified by HPLC-MS/MS in the samples treated with microwave. The frequencies of proteins changed after microwaves treatment were labeled as 39.13% (synthesis and metabolism of amino acid or proteins), 21.74% (carbohydrate metabolism), 8.70% (anti-oxidant and acetyl Co-A synthesis), and 4.35% (the catalyst of catabolism of bacterial acetoin, ethanol metabolism, glyoxylate pathway, butyrate synthesis and detoxification activity), respectively. This study indicates that microwaves result in the inactivation of Bacillus cereus by cleaning nuclear chromatin, disrupting cell membrane and disordering the expression of proteins.

Keywords: Bacillus cereus; microwave; morphology; proteomics; the permeability of membrane.

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Figures

Figure 1
Figure 1
The scanning electron microscope (SEM) photography of Bacillus cereus in control group (× 1,500) (A) and microwave treatments group (× 1,500) (B), respectively.
Figure 2
Figure 2
The transmission electron microscope (TEM) photography of Bacillus cereus in control group (× 2,500) (A) and microwave treatments group (× 2,500) (B), respectively.
Figure 3
Figure 3
2-DE pattern of total proteins of Bacillus cereus in control group (A) and microwave treatments group (B), respectively.
Figure 4
Figure 4
Distribution of the proteins spots identified among different classes by biological process.
See this image and copyright information in PMC

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