Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Aug 31:9:2044.
doi: 10.3389/fmicb.2018.02044. eCollection 2018.

Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital

Chendi Zhu  1 , Veranja Liyanapathirana  2 , Carmen Li  1 , Vasanthi Pinto  3 , Mamie Hui  1 , Norman Lo  1 , Kam T Wong  1 , Nilanthi Dissanayake  2 , Margaret Ip  1
Affiliations

Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital

Chendi Zhu et al. Front Microbiol. .

Abstract

Limited data is available on the epidemiology and characteristics of carbapenem-resistant Enterobacteriaceae (CRE) and their associated plasmids or virulence determinants from Sri Lanka. Through whole genome sequencing of CREs from the intensive care units of a Sri Lankan teaching hospital, we identified a carbapenemase gene, blaOXA-181 in 10 carbapenemase-producing Klebsiella pneumoniae isolates (two strains of ST437 and eight strains of ST147) from 379 respiratory specimens. blaOXA-181 was carried in three variants of ColE-type plasmids. K. pneumoniae strains with ompK36 variants showed high minimum inhibitory concentrations to carbapenem. Furthermore, genes encoding for extended spectrum β-lactamases (ESBL), plasmid-mediated quinolone resistance (PMQR) determinants (qnr, aac(6')-Ib-cr, and oqxAB) were present in all 10 strains. Amino acid substitution in chromosomal quinolone resistance-determining regions (QRDRs) gyrA (Ser83Ile) and parC (Ser80Ile) were also observed. All strains had yersiniabactin genes on mobile element ICEkp. Strict infection control practices and judicious use of antibiotics are warranted to prevent further spread of multidrug-resistant K. pneumoniae.

Keywords: Klebsiella pneumoniae; OXA-181; Sri Lanka; quinolone; yersiniabactin.

PubMed Disclaimer

Figures

FIGURE 1
FIGURE 1
Pan-genome dendrogram of 10 Sri Lanka strains with annotation. ∗Red boxes indicate presence and yellow for absence.
FIGURE 2
FIGURE 2
ColE-type plasmids in Sri Lanka. ∗CUHK_SL-B had ISEcp1 deletion; CUHK_SL-C had ISEcp1 and mobC deletion.
See this image and copyright information in PMC

References

    1. Aldred K. J., Kerns R. J., Osheroff N. (2014). Mechanism of quinolone action and resistance. Biochemistry 53 1565–1574. 10.1021/bi5000564 - DOI - PMC - PubMed
    1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. (1990). Basic local alignment search tool. J. Mol. Biol. 215 403–410. 10.1016/S0022-2836(05)80360-2 - DOI - PubMed
    1. Andrews S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data. Available at: http://www.bioinformatics.babraham.ac.uk/projects/fastqc/
    1. Bankevich A., Nurk S., Antipov D., Gurevich A. A., Dvorkin M., Kulikov A. S., et al. (2012). SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J. Comput. Biol. 19 455–477. 10.1089/cmb.2012.0021 - DOI - PMC - PubMed
    1. Carattoli A., Zankari E., García-Fernández A., Voldby Larsen M., Lund O., Villa L., et al. (2014). In silico detection and typing of plasmids using plasmidfinder and plasmid multilocus sequence typing. antimicrob. Agents Chemother. 58 3895–3903. 10.1128/AAC.02412-14 - DOI - PMC - PubMed

LinkOut - more resources