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. 2018 Oct;8(10):406.
doi: 10.1007/s13205-018-1430-9. Epub 2018 Sep 14.

The draft genome sequence of Clostridium sp. strain LJ4 with high furan and phenolic derivates' tolerances occurring from lignocellulosic hydrolysates

Jie Liu  1 , Yujia Jiang  1 , Tianpeng Chen  1 , Weiliang Dong  1   2 , Wenming Zhang  1   2 , Jiangfeng Ma  1   2 , Min Jiang  1   2 , Fengxue Xin  1   2
Affiliations

The draft genome sequence of Clostridium sp. strain LJ4 with high furan and phenolic derivates' tolerances occurring from lignocellulosic hydrolysates

Jie Liu et al. 3 Biotech. 2018 Oct.

Abstract

The genome of a wild-type solventogenic Clostridium sp. strain LJ4 that could directly convert undetoxified lignocellulosic hydrolysate to butanol and tolerate high concentration of furan and phenolic derivates occurring in the lignocellulosic hydrolysate is described. 16S rDNA gene sequencing and analysis indicated that it is closely related to Clostridium acetobutylicum. The genome size of strain LJ4 is 3.90 Mp, which has a G + C content of 30.72% and encodes 2711 proteins. It also has one 0.19 Mp plasmid with 181 predicted encoding proteins. Alcohol dehydrogenases (ADs) and a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent flavin mononucleotide (FMN) reductase were identified, which may play key roles in inhibitors' resistance in lignocellulosic hydrolysate.

Keywords: Alcohol dehydrogenases; Clostridium; Draft genome sequencing; Furan and phenolic derivates; NADPH-dependent FMN reductase.

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Conflict of interest statement

Compliance with ethical standardsThe authors have declared there was no conflict of interest.

Figures

Fig. 1
Fig. 1
Phylogenetic tree of Clostridium sp. strain LJ4 using the neighbor joining method (MEGA5.01) based on its 16S rDNA gene sequences. The taxon named after strain and appended accession number in brackets and the strain with a T for Type Culture in superscript. The scale represents the genetic distance between sequences. Numbers displayed on the branches are the bootstrap support obtained through 1000. The full names of the abbreviations for the culture collections: ATCC American Type Culture Collection, NCIMB National Collections of Industrial, Food and Marine Bacterial, DSM Deutsche Sammlung von Mikroorganismen und Zellkulturen, JCM Japan Collection of Microorganisms Replications
Fig. 2
Fig. 2
Neighbor joining tree calculated by ClustalW for a gene 3-352 Alcohol dehydrogenases, b gene 3-409 A nicotinamide adenine dinucleotide phosphate (NADPH)-dependent flavin mononucleotide from Clostridium sp. LJ4 constructed by MEGA 5.01. The taxon named after enzyme and appended accession number in brackets. The scale represents the genetic distance between sequences. Numbers displayed on the branches are the bootstrap support obtained through 1000 replications
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