Nonhomologous recombination in the parvovirus chromosome: role for a CTATTTCT motif
- PMID: 3023957
- PMCID: PMC367873
- DOI: 10.1128/mcb.6.8.3005-3009.1986
Nonhomologous recombination in the parvovirus chromosome: role for a CTATTTCT motif
Abstract
The mechanism of nonhomologous recombination in murine cells infected with the parvovirus minute virus of mice (MVM) has been investigated by analysis of DNA sequences at recombination junctions in naturally occurring deletion variants of the virus. We report here that nonhomologous recombination in the MVM chromosome is characterized by short homologies, by insertion at recombination junctions of foreign DNA sequences that are enriched for preferred eucaryotic topoisomerase I cleavage sites, and by an association with a common DNA sequence motif of the type 5'-CTATTTCT-3'. Additional analyses of broken MVM chromosomes provided evidence for specific enzymatic cleavage within 5'-CTTATC-3' and 5'-CTATTC-3' sequences. The results indicate that the 5'-CTATTTCT-3' motif is an important genetic element for nonhomologous recombination in the parvovirus chromosome.
Similar articles
-
Short direct repeats mediate spontaneous high-frequency deletions in DNA of minute virus of mice.Mol Cell Biol. 1984 Oct;4(10):2239-42. doi: 10.1128/mcb.4.10.2239-2242.1984. Mol Cell Biol. 1984. PMID: 6095052 Free PMC article.
-
cis-acting sequences in the Aleutian mink disease parvovirus late promoter important for transcription: comparison to the canine parvovirus and minute virus of mice.J Virol. 1993 Apr;67(4):1887-95. doi: 10.1128/JVI.67.4.1887-1895.1993. J Virol. 1993. PMID: 8383216 Free PMC article.
-
An Escherichia coli recBCsbcBrecF host permits the deletion-resistant propagation of plasmid clones containing the 5'-terminal palindrome of minute virus of mice.Gene. 1985;35(1-2):179-85. doi: 10.1016/0378-1119(85)90170-2. Gene. 1985. PMID: 3896934
-
Association of crossover points with topoisomerase I cleavage sites: a model for nonhomologous recombination.Science. 1985 Nov 22;230(4728):954-8. doi: 10.1126/science.2997924. Science. 1985. PMID: 2997924
-
Sequences and structures at hepadnaviral integration: recombination sites implicate topoisomerase I in hepadnaviral DNA rearrangements and integration.J Hepatol. 1995;22(1 Suppl):21-33. J Hepatol. 1995. PMID: 7602072 Review.
Cited by
-
Gene deletions causing human genetic disease: mechanisms of mutagenesis and the role of the local DNA sequence environment.Hum Genet. 1991 Mar;86(5):425-41. doi: 10.1007/BF00194629. Hum Genet. 1991. PMID: 2016084
-
Within-host genetic diversity of endemic and emerging parvoviruses of dogs and cats.J Virol. 2008 Nov;82(22):11096-105. doi: 10.1128/JVI.01003-08. Epub 2008 Sep 3. J Virol. 2008. PMID: 18768982 Free PMC article.
-
Compilation of DNA strand exchange sites for non-homologous recombination in somatic cells.Nucleic Acids Res. 1988 Mar 11;16(5):1739-58. doi: 10.1093/nar/16.5.1739. Nucleic Acids Res. 1988. PMID: 2832826 Free PMC article.
-
A mechanism for deletion formation in DNA by human cell extracts: the involvement of short sequence repeats.Nucleic Acids Res. 1992 Dec 11;20(23):6183-8. doi: 10.1093/nar/20.23.6183. Nucleic Acids Res. 1992. PMID: 1475181 Free PMC article.
-
Mapping in vivo topoisomerase I sites on simian virus 40 DNA: asymmetric distribution of sites on replicating molecules.Mol Cell Biol. 1989 Feb;9(2):541-50. doi: 10.1128/mcb.9.2.541-550.1989. Mol Cell Biol. 1989. PMID: 2540421 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
