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. 1986 Nov 11;14(21):8605-13.
doi: 10.1093/nar/14.21.8605.

Nucleotide sequence of the cellulase gene celD encoding endoglucanase D of Clostridium thermocellum

G Joliff, P Béguin, J P Aubert
Free PMC article

Nucleotide sequence of the cellulase gene celD encoding endoglucanase D of Clostridium thermocellum

G Joliff et al. Nucleic Acids Res. .
Free PMC article

Abstract

The nucleotide sequence of the celD gene, encoding the previously crystallized endoglucanase D of Clostridium thermocellum, is reported. The enzyme shares a conserved, reiterated domain with the COOH-terminal end of endoglucanases A and B from the same organism. The overexpression in Escherichia coli of celD subcloned in pUC8 appears to result from a translational fusion of the NH2-terminal end of the endoglucanase with the NH2-terminal end of beta-galactosidase.

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