doi: 10.1093/nar/14.21.8637.
Nucleotide sequence and analysis of the 58.3 to 65.5-kb early region of bacteriophage T4
- PMID: 3024113
- PMCID: PMC311883
- DOI: 10.1093/nar/14.21.8637
Item in Clipboard
Nucleotide sequence and analysis of the 58.3 to 65.5-kb early region of bacteriophage T4
Nucleic Acids Res.
.
Free PMC article
Abstract
The complete 7.2-kb nucleotide sequence from the 58.3 to 65.5-kb early region of bacteriophage T4 has been determined by Maxam and Gilbert sequencing. Computer analysis revealed at least 20 open reading frames (ORFs) within this sequence. All major ORFs are transcribed from the left strand, suggesting that they are expressed early during infection. Among the ORFs, we have identified the ipIII, ipII, denV and tk genes. The ORFs are very tightly spaced, even overlapping in some instances, and when ORF interspacing occurs, promoter-like sequences can be implicated. Several of the sequences preceding the ORFs, in particular those at ipIII, ipII, denV, and orf61.9, can potentially form stable stem-loop structures.
Similar articles
-
Identification, physical map location and sequence of the denV gene from bacteriophage T4.Nucleic Acids Res. 1984 Nov 12;12(21):8085-96. doi: 10.1093/nar/12.21.8085. Nucleic Acids Res. 1984. PMID: 6095188 Free PMC article.
-
[Nucleotide sequence of gene 31 of the T4 bacteriophage].Mol Biol (Mosk). 1988 Nov-Dec;22(6):1507-16. Mol Biol (Mosk). 1988. PMID: 2855254 Russian.
-
Analysis of five presumptive protein-coding sequences clustered between the primosome genes, 41 and 61, of bacteriophages T4, T2, and T6.J Virol. 1993 Apr;67(4):2305-16. doi: 10.1128/JVI.67.4.2305-2316.1993. J Virol. 1993. PMID: 8383243 Free PMC article.
-
Gene rIII is the nearest downstream neighbour of bacteriophage T4 gene 31.Gene. 1992 May 1;114(1):85-90. doi: 10.1016/0378-1119(92)90711-w. Gene. 1992. PMID: 1587487
-
Sequence and transcripts of the bacteriophage T4 DNA repair gene uvsY.Genetics. 1986 Dec;114(4):1061-79. doi: 10.1093/genetics/114.4.1061. Genetics. 1986. PMID: 3026891 Free PMC article.
Cited by
-
Quaternary structure of vaccinia virus thymidine kinase.Biochem Biophys Res Commun. 1990 Jun 29;169(3):1080-6. doi: 10.1016/0006-291x(90)92005-k. Biochem Biophys Res Commun. 1990. PMID: 2114104 Free PMC article.
-
Bacteriophage T4 genome.Microbiol Mol Biol Rev. 2003 Mar;67(1):86-156, table of contents. doi: 10.1128/MMBR.67.1.86-156.2003. Microbiol Mol Biol Rev. 2003. PMID: 12626685 Free PMC article. Review.
-
Structure of the N-terminal region of Haemophilus influenzae H10017: implications for function.J Biomol NMR. 2001 Jun;20(2):105-10. doi: 10.1023/a:1011264300726. J Biomol NMR. 2001. PMID: 11495242
-
Prediction of functional class of novel viral proteins by a statistical learning method irrespective of sequence similarity.Virology. 2005 Jan 5;331(1):136-43. doi: 10.1016/j.virol.2004.10.020. Virology. 2005. PMID: 15582660 Free PMC article.
-
The sequences and activities of RegB endoribonucleases of T4-related bacteriophages.Nucleic Acids Res. 2004 Oct 14;32(18):5582-95. doi: 10.1093/nar/gkh892. Print 2004. Nucleic Acids Res. 2004. PMID: 15486207 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
