Cloning of cDNA for human T-cell replacing factor (interleukin-5) and comparison with the murine homologue
- PMID: 3024129
- PMCID: PMC311935
- DOI: 10.1093/nar/14.22.9149
Cloning of cDNA for human T-cell replacing factor (interleukin-5) and comparison with the murine homologue
Abstract
We have cloned cDNA for T-cell replacing factor (interleukin-5), which replaces T-cell helper function for normal B cells which secrete immunoglobulin, from human T cell leukemia line, ATL-2, using mouse interleukin-5 cDNA as probe. Total nucleotide sequence of the cDNA (816 base pairs) was determined and compared with that of mouse interleukin-5 cDNA. The cloned cDNA encoded the interleukin-5 precursor of 134 amino acids containing an N-terminal signal sequence. Although the human interleukin-5 precursor is one amino acid longer than the murine homologue, the sizes of the mature proteins appear similar. The nucleotide and amino acid sequence homologies of the coding regions of human and murine interleukin-5 are 77% and 70%, respectively. Human interleukin-5 synthesized by the direction of the cloned cDNA induced immunoglobulin synthesis in human B cells stimulated by Staphylococcus aureus mitogen.
Similar articles
-
Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities.Proc Natl Acad Sci U S A. 1986 Aug;83(16):5894-8. doi: 10.1073/pnas.83.16.5894. Proc Natl Acad Sci U S A. 1986. PMID: 3016727 Free PMC article.
-
Isolation and characterization of lymphokine cDNA clones encoding mouse and human IgA-enhancing factor and eosinophil colony-stimulating factor activities: relationship to interleukin 5.Proc Natl Acad Sci U S A. 1987 Nov;84(21):7388-92. doi: 10.1073/pnas.84.21.7388. Proc Natl Acad Sci U S A. 1987. PMID: 2823259 Free PMC article.
-
Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter.Nature. 1986 Feb 20-26;319(6055):640-6. doi: 10.1038/319640a0. Nature. 1986. PMID: 3005865
-
Molecular cloning and structure of the mouse interleukin-5 gene.Growth Factors. 1988;1(1):51-7. doi: 10.3109/08977198809000246. Growth Factors. 1988. PMID: 3078564
-
The murine homologue of the T lymphocyte CD2 antigen: molecular cloning, chromosome assignment and cell surface expression.Eur J Immunol. 1987 Jul;17(7):1015-20. doi: 10.1002/eji.1830170718. Eur J Immunol. 1987. PMID: 2440689
Cited by
-
Interleukin 4 inhibits the proliferation but not the differentiation of activated human B cells in response to interleukin 2.J Exp Med. 1988 Oct 1;168(4):1321-37. doi: 10.1084/jem.168.4.1321. J Exp Med. 1988. PMID: 3262709 Free PMC article.
-
Molecular cloning and expression of the murine interleukin-5 receptor.EMBO J. 1990 Dec;9(13):4367-74. doi: 10.1002/j.1460-2075.1990.tb07886.x. EMBO J. 1990. PMID: 2265612 Free PMC article.
-
Activation of T cell-derived lymphokine genes in T cells and fibroblasts: effects of human T cell leukemia virus type I p40x protein and bovine papilloma virus encoded E2 protein.Nucleic Acids Res. 1988 Jul 25;16(14A):6547-66. doi: 10.1093/nar/16.14.6547. Nucleic Acids Res. 1988. PMID: 2840644 Free PMC article.
-
B-cell stimulatory factors (BSFs): molecular structure, biological function, and regulation of expression.J Clin Immunol. 1987 Sep;7(5):343-55. doi: 10.1007/BF00917012. J Clin Immunol. 1987. PMID: 3308942 Review. No abstract available.
-
Triple synergism of human T-lymphotropic virus type 1-encoded tax, GATA-binding protein, and AP-1 is required for constitutive expression of the interleukin-5 gene in adult T-cell leukemia cells.Mol Cell Biol. 1997 Aug;17(8):4272-81. doi: 10.1128/MCB.17.8.4272. Mol Cell Biol. 1997. PMID: 9234684 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases