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. 2018:1850:123-131.
doi: 10.1007/978-1-4939-8730-6_9.

CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors

David L Hacker  1 , Laurence Durrer  2 , Soraya Quinche  2
Affiliations

CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors

David L Hacker et al. Methods Mol Biol. 2018.

Abstract

Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the two most popular mammalian hosts for the production of recombinant proteins. In this chapter the suspension cultivation and transfection of these cells in small-scale disposable bioreactors is described. The TubeSpin bioreactor 50 and TubeSpin bioreactor 600 are designed for the cultivation of suspension cells by orbital shaking and have maximum working volumes of about 15 mL and 400 mL, respectively.

Keywords: CHO cells; Disposable bioreactors; HEK293 cells; Suspension cells; Transfection.

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