Herpesvirus (pseudorabies virus) latency in swine: occurrence and physical state of viral DNA in neural tissues

Abstract

The occurrence of the pseudorabies virus (PRV, herpes suis 1) genome in various neural tissues of latently infected pigs was investigated. During the latent phase of infection, between 7 and 52 weeks p.i., the average amount of PRV DNA ranged between 0.3 and 0.05 genome copies per cell. The results obtained by in situ cytohybridization and reassociation kinetic experiments indicated that each latently infected cell harbored at least 30 viral genome copies. PRV DNA could be demonstrated in similar frequencies (about 30% of cases) in the trigeminal ganglia, the olfactory bulb, and the medulla oblongata, and less frequently in the brain stem and the spinal cord. Southern blot analysis showed that in general the physical state of the latent genome was linear and nonintegrated. Only in 2 of 15 animals could the presence of circular or concatemeric viral DNA be observed. Thus, we could show that over a period of 13 months after infection the PRV genome persisted both qualitatively and quantitatively in a stable state in different areas of both the peripheral and the central nervous system.