Detection of extracellular matrix antigens (fibronectin, laminin, type IV in collagen) in paraffin embedded sections by avidin-biotin-peroxidase complex labelling
- PMID: 3024614
Item in Clipboard
Detection of extracellular matrix antigens (fibronectin, laminin, type IV in collagen) in paraffin embedded sections by avidin-biotin-peroxidase complex labelling
Basic Appl Histochem.
1986.
Abstract
An avidin-biotin-peroxidase labelling method was applied to frozen sections and to routine-fixed, paraffin-embedded sections, and compared with immunohistochemical results of stromal antigen detection (type IV collagen, laminin and fibronectin) obtained in parallel on different neoplasias. Lung, breast and gastrointestinal tract tumors were studied. Superimposable sections were obtained from cryostat and formalin-fixed, paraffin-embedded specimens (previously digested by pepsin). The data demonstrate the potential use of immunohistochemical investigation of paraffin-embedded tissues for histological analyses of tumors.
Similar articles
-
Distribution of laminin, collagen type IV, collagen type I, and fibronectin in chicken cardiac jelly/basement membrane.Anat Rec. 1989 Jul;224(3):417-25. doi: 10.1002/ar.1092240310. Anat Rec. 1989. PMID: 2675672
-
Immunostaining for leukocyte common antigen using an amplified avidin-biotin-peroxidase complex method and paraffin sections. A study of 735 hematopoietic and nonhematopoietic human neoplasms.Arch Pathol Lab Med. 1987 Nov;111(11):1035-9. Arch Pathol Lab Med. 1987. PMID: 2444189
-
Distribution of type IV collagen, laminin, and fibronectin during maxillary process formation in the chick embryo.Am J Anat. 1990 Mar;187(3):232-46. doi: 10.1002/aja.1001870303. Am J Anat. 1990. PMID: 2181854
-
The hepatic extracellular matrix. II. Electron immunohistochemical studies in rats with CCl4-induced cirrhosis.Lab Invest. 1985 Aug;53(2):166-86. Lab Invest. 1985. PMID: 3894794 Review.
-
Expression and potential role of the extracellular matrix in hepatic ontogenesis: a review.Microsc Res Tech. 1997 Nov 15;39(4):372-86. doi: 10.1002/(SICI)1097-0029(19971115)39:4<372::AID-JEMT7>3.0.CO;2-J. Microsc Res Tech. 1997. PMID: 9407547 Review.
Cited by
-
Evaluation of hepatocellular carcinoma aggressiveness by a panel of extracellular matrix antigens.Am J Pathol. 1991 Mar;138(3):647-54. Am J Pathol. 1991. PMID: 1848041 Free PMC article.
-
Immunohistochemical study of basement membrane antigens in bronchioloalveolar carcinoma.Am J Pathol. 1987 Aug;128(2):217-24. Am J Pathol. 1987. PMID: 3039848 Free PMC article.
-
Changes in the alveolar connective tissue of the ageing lung. An immunohistochemical study.Virchows Arch A Pathol Anat Histopathol. 1989;415(2):137-44. doi: 10.1007/BF00784351. Virchows Arch A Pathol Anat Histopathol. 1989. PMID: 2500769