Microbispora triticiradicis sp. nov., a novel actinomycete isolated from the root of wheat (Triticum aestivum L.)

Abstract

A novel actinomycete, designated strain NEAU-HRDPA2-9^T, was isolated from the roots of wheat (Triticumaestivum L.) and characterized using a polyphasic approach. The morphological and chemotaxonomic characteristics of the strain coincided with those of members of the genus Microbispora. The 16S rRNA gene sequence analysis showed that the isolate was most closely related to Microbispora bryophytorum NEAU-TX2-2^T (98.6 %), Microbispora hainanensis DSM 45428^T (98.5 %), Microbispora camponoti 2C-HV3^T (98.5 %), Microbispora amethystogenes JCM 3021^T (98.2 %), Microbispora siamensis NBRC 104113^T (98.1 %), Microbispora corallina JCM 10267^T (98.0 %) and Microbispora roseasubsp.rosea JCM 3006^T (97.9 %). However, two tree-making algorithms supported the position that strain NEAU-HRDPA2-9^T formed a distinct clade with M. siamensis NBRC 104113^T and M. hainanensis DSM 45428^T. Furthermore, a combination of DNA-DNA hybridization results and some physiological and biochemical properties demonstrated that the strain could be distinguished from its closest relatives. Therefore, it is proposed that strain NEAU-HRDPA2-9^T should be classified as representative of a novel species of the genus Microbispora, for which the name Microbisporatriticiradicis sp. nov. is proposed. The type strain is NEAU-HRDPA2-9^T (=CGMCC 4.7399^T=DSM 104649^T).

Keywords: 16S rRNA gene; Microbispora triticiradicis sp. nov.; polyphasic analysis.