High stoichiometric food quality increases moulting organism vulnerability to pollutant impacts: An experimental test with Gammarus fossarum (Crustacea: Amphipoda)

Abstract

Headwater organisms are most often simultaneously faced with multiple stressors such as low resource quality and pollutants. Higher food quality has been hypothesized to enhance the tolerance of organisms to pollutants, but the interactive effects of food quality and pollutants on species and ecosystems remain poorly studied. To better understand these interactive effects, we experimentally manipulated the phosphorus (P) content of two leaf litters with contrasted carbon quality (alder and maple). During four weeks, individuals of the detritivorous crustacean Gammarus fossarum were exposed to low levels of cadmium ([Cd] = 0, 0.35 or 0.70 μg L^-1) while being fed with one of the leaf P treatments. When organisms were not exposed to Cd, their high survival rate was more driven by the carbon quality of the resource (litter species) than by its stoichiometric quality. In contrast, their number of moults and growth rates were primarily increased by the P content of resources. When exposed to Cd, G. fossarum survival rate was reduced, but this effect was largely magnified by a higher P level in resources. Our results showed that despite positive effects of resource stoichiometric quality on organism life history traits (growth, survival), a resource of high stoichiometric quality might be detrimental for organisms exposed to low and environmentally realistic levels of pollutants. Two non-exclusive hypotheses are proposed to explain these results. First, organisms fed on the highest quality resource exhibited the highest moulting frequencies (moults being the most critical life cycle step of arthropods), which could have rendered them more sensitive to pollutants. Secondly, the metabolism of organisms fed on higher quality resources was potentially enhanced, increasing the uptake of dissolved Cd by gammarids. This study suggests that species sensitivity to pollutants might be underestimated in ecosystems facing both nutrient constraint and pollutants.

Keywords: Cadmium; Ecological stoichiometry; Gammarus fossarum; Growth; Moult; Survival.