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. 2018 Sep 21;8(10):751.
doi: 10.3390/nano8100751.

Antibacterial Activity of Fructose-Stabilized Silver Nanoparticles Produced by Direct Current Atmospheric Pressure Glow Discharge towards Quarantine Pests

Anna Dzimitrowicz  1 , Agata Motyka-Pomagruk  2 , Piotr Cyganowski  3 , Weronika Babinska  4 , Dominik Terefinko  5 , Piotr Jamroz  6 , Ewa Lojkowska  7 , Pawel Pohl  8 , Wojciech Sledz  9
Affiliations

Antibacterial Activity of Fructose-Stabilized Silver Nanoparticles Produced by Direct Current Atmospheric Pressure Glow Discharge towards Quarantine Pests

Anna Dzimitrowicz et al. Nanomaterials (Basel). .

Abstract

Development of efficient plant protection methods against bacterial phytopathogens subjected to compulsory control procedures under international legislation is of the highest concern having in mind expensiveness of enforced quarantine measures and threat of the infection spread in disease-free regions. In this study, fructose-stabilized silver nanoparticles (FRU-AgNPs) were produced using direct current atmospheric pressure glow discharge (dc-APGD) generated between the surface of a flowing liquid anode (FLA) solution and a pin-type tungsten cathode in a continuous flow reaction-discharge system. Resultant spherical and stable in time FRU-AgNPs exhibited average sizes of 14.9 ± 7.9 nm and 15.7 ± 2.0 nm, as assessed by transmission electron microscopy (TEM) and dynamic light scattering (DLS), respectively. Energy dispersive X-ray spectroscopy (EDX) analysis revealed that the obtained nanomaterial was composed of Ag while selected area electron diffraction (SAED) indicated that FRU-AgNPs had the face-centered cubic crystalline structure. The fabricated FRU-AgNPs show antibacterial properties against Erwinia amylovora, Clavibacter michiganensis, Ralstonia solanacearum, Xanthomonas campestris pv. campestris and Dickeya solani strains with minimal inhibitory concentrations (MICs) of 1.64 to 13.1 mg L-1 and minimal bactericidal concentrations (MBCs) from 3.29 to 26.3 mg L-1. Application of FRU-AgNPs might increase the repertoire of available control procedures against most devastating phytopathogens and as a result successfully limit their agricultural impact.

Keywords: Clavibacter michiganensis; Dickeya solani; Erwinia amylovora; Ralstonia solanacearum; Xanthomonas campestris pv. campestris; atmospheric pressure plasma; nanostructures; phytopathogens; plant protection; quarantine.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
A schematic representation of the dc-APGD-based reaction-discharge system working in a continuous-flow mode. (1) A pin-type W cathode, (2) dc-APGD, (3) a graphite tube, (4) a working solution (with the AgNPs precursor and d-fructose) acting as a flowing liquid anode (FLA), (5) a quartz capillary, and (6) a compartment for the collection of the dc-APGD treated working solution containing the synthesized FRU-AgNPs.
Figure 2
Figure 2
The UV/Vis absorption spectrum of five times diluted colloidal suspension of FRU-AgNPs.
Figure 3
Figure 3
TEM micrographs illustrating shapes and size distribution of FRU-AgNPs.
Figure 4
Figure 4
Granulometric properties of Ag nanostructures (A) The SAED pattern for the micrograph of FRU-AgNPs and (B) the EDX spectrum for the presented FRU-AgNPs.
Figure 5
Figure 5
Percentage size distribution by the number of FRU-AgNPs estimated by dynamic light scattering (DLS).
Figure 6
Figure 6
ATR FT-IR spectra of the working solution before (FRU-AgNPs precursor) and after (FRU-AgNPs) dc-APGD treatment.
See this image and copyright information in PMC

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