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. 2018 Oct 17;38(5):BSR20181113.
doi: 10.1042/BSR20181113. Print 2018 Oct 31.

Biopanning of allergens from wasp sting patients

Lin Chai  1 , Xianyi Yang  1 , Mei Liu  1 , Chunyan Liu  2 , Limei Han  3 , Hui Guo  1 , Changsheng Li  1 , Yuwen Sun  1 , Xiaoyan Li  1 , Min Xiao  4 , Zhicheng Fang  4
Affiliations

Biopanning of allergens from wasp sting patients

Lin Chai et al. Biosci Rep. .

Abstract

Objective: Wasp venom is a potentially important natural drug, but it can cause hypersensitivity reactions. The purpose of the present study was to systematically study the epitopes of wasp venom.

Methods: Using a random 12-peptide phage library, we performed antibody-binding epitope panning on ten serum samples from wasp sting victims at 3 h and 4 days after the sting. The panning epitopes were identified by high-throughput sequencing and matched with wasp venom proteins by BLAST. The panned antibody-binding epitopes were verified by ELISA.

Results: A total of 35 specific potential wasp venom epitopes in 4 days were identified. Amongst them, twelve peptide epitopes were matched with nine wasp venom proteins, namely, vitellogenin precursor, hexamerin 70b precursor, venom carboxylesterase-6 precursor, MRJP5, major royal jelly protein 8 precursor, venom acid phosphatase Acph-1 precursor, phospholipase A2, venom serine protease 34 precursor, and major royal jelly protein 9 precursor. The changes in serum IgM antibodies induced by wasp venom were confirmed by ELISA based on the 12 peptide epitopes.

Conclusion: The nine wasp venom proteins are potential allergens, which should be excluded or modified in the potential biomedical applications of wasp venom.

Keywords: allergens; peptide; wasp venom.

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Conflict of interest statement

The authors declare that there are no competing interests associated with the manuscript.

Figures

Figure 1
Figure 1. ELISA detection of the IgM in serum of patients stung by wasp after 3 h or 4 days with 12 antigenic epitopes
**P<0.01.
See this image and copyright information in PMC

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