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. 2018 Aug;11(8):1196-1202.
doi: 10.14202/vetworld.2018.1196-1202. Epub 2018 Aug 30.

Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

Dela Ayu Lestari  1 , Endang Purbowati  1 , Sutopo Sutopo  1 , Edy Kurnianto  1
Affiliations

Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

Dela Ayu Lestari et al. Vet World. 2018 Aug.

Abstract

Aim: This study aimed to analyze the amino acid sequence of Cytochrome b (Cyt b) gene in Kejobong goat and its genetic relationships with local goats located in Asia.

Materials and methods: A total of 28 heads of Kejobong goat were purposively sampled. The deoxyribonucleic acid (DNA) was extracted from blood using gSYNC DNA mini kit (Geneaid Biotech Ltd.). Cyt b gene was amplified using polymerase chain reaction (PCR) method with CytbCapF and CytbCapR primers. The amplified PCR products were sequenced for further analysis.

Results: There were a total 377 amino acid sequences translated from 1140 base pair (bp) of Cyt b gene, 99.20% of it were monomorphic, amino acid alterations were found at site 16th, 121st, and 231st, and Kejobong goat was in the same cluster with Southeast Asian local goats.

Conclusion: Most of the amino acid sequence on Cyt b gene in Kejobong goat is monomorphic (99.20%), only a few nucleotide mutations were found that causing amino acid alteration in three sites (0.80%). Kejobong goat has a close genetic relationship to several local goats in Southeast Asian.

Keywords: Kejobong goat; amino acid sequence; cytochrome b.

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Figures

Figure-1
Figure-1
Polymerase chain reaction amplification results of Cyt b gene in Kejobong goat.
Figure-2
Figure-2
Amino acid sequence from site 1 to 95 of Cyt b gene in Kejobong goat.
Figure-3
Figure-3
Amino acid sequence from site 96 to 190 of Cyt b gene in Kejobong goat.
Figure-4
Figure-4
Amino acid sequence from site 191 to 285 of Cyt b gene in Kejobong goat.
Figure-5
Figure-5
Amino acid sequence from site 286 to 377 of Cyt b gene in Kejobong goat.
Figure-6
Figure-6
Percentage of amino acid sequence of Cyt b gene on Kejobong goat.
Figure-7
Figure-7
Phylogeny tree of Kejobong goat and several Asian local goats based on amino acid sequence.
Figure-8
Figure-8
Mutation point of Cyt b gene in Kejobong goat.
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References

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