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Review
. 2018 Sep 10:9:2087.
doi: 10.3389/fmicb.2018.02087. eCollection 2018.

Recent Advances on the Multiplex Molecular Detection of Plant Viruses and Viroids

Vicente Pallás  1 , Jesus A Sánchez-Navarro  1 , Delano James  2
Affiliations
Review

Recent Advances on the Multiplex Molecular Detection of Plant Viruses and Viroids

Vicente Pallás et al. Front Microbiol. .

Abstract

Plant viruses are still one of the main contributors to economic losses in agriculture. It has been estimated that plant viruses can cause as much as 50 billion euros loss worldwide, per year. This situation may be worsened by recent climate change events and the associated changes in disease epidemiology. Reliable and early detection methods are still one of the main and most effective actions to develop control strategies for plant viral diseases. During the last years, considerable progress has been made to develop tools with high specificity and low detection limits for use in the detection of these plant pathogens. Time and cost reductions have been some of the main objectives pursued during the last few years as these increase their feasibility for routine use. Among other strategies, these objectives can be achieved by the simultaneous detection and (or) identification of several viruses in a single assay. Nucleic acid-based detection techniques are especially suitable for this purpose. Polyvalent detection has allowed the detection of multiple plant viruses at the genus level. Multiplexing RT polymerase chain reaction (PCR) has been optimized for the simultaneous detection of more than 10 plant viruses/viroids. In this short review, we provide an update on the progress made during the last decade on techniques such as multiplex PCR, polyvalent PCR, non-isotopic molecular hybridization techniques, real-time PCR, and array technologies to allow simultaneous detection of multiple plant viruses. Also, the potential and benefits of the powerful new technique of deep sequencing/next-generation sequencing are described.

Keywords: microarrays; molecular hybridization; multiplex; nextgeneration sequencing; plant viruses; polymerase chain reaction; polyprobes; viroids.

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Figures

FIGURE 1
FIGURE 1
References of multiplex detection assays in the period 2005–2018. Number of references that use RT-PCR (yellow), real-time RT-PCR SYBR (green), and real-time RT-PCR TaqMan (light green) are represented against the number of pathogens detected by these techniques. Source used was the Web of Science (all database) and the parameters used for the searching were: (multiplex or simultaneous or polyvalent) and detection and (plant virus or viroid).
See this image and copyright information in PMC

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