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. 1986 Dec;103(6 Pt 2):2629-36.
doi: 10.1083/jcb.103.6.2629.

Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor

M G Waters, W J Chirico, G Blobel

Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor

M G Waters et al. J Cell Biol. 1986 Dec.

Abstract

We have found that a soluble activity present in the postribosomal supernatant fraction of Saccharomyces cerevisiae stimulates posttranslational translocation of yeast prepro-alpha-factor across yeast microsomal membranes. Stimulation of translocation is not due to a nonspecific affect on ATP levels. The activity is likely to be due to protein(s) as it is destroyed by N-ethylmaleimide, protease, or heat treatment but not by incubation with RNase. Its apparent sedimentation coefficient is approximately 9.6 S.

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