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. 1986 Nov;14(3-4):229-35.
doi: 10.1016/0166-0934(86)90024-8.

An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy

A A Codd, H K Narang

An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy

A A Codd et al. J Virol Methods. 1986 Nov.

Abstract

Faecal specimens from 520 patients with non-bacterial, gastroenteritis were examined by electron microscopy using four methods. These were (1) a direct dip method, (2) low-speed centrifugation, (3) ultracentrifugation and (4) a calcium phosphate method. The calcium phosphate method combined with low-speed centrifugation (750 X g, 2,100 X g) was considered overall best. The calcium phosphate method makes it possible to handle a large number of faecal specimens by saving considerable time and labour.

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