Comparative Study
doi: 10.1016/0166-0934(86)90035-2.
Two enzyme immunoassays for the detection of antibody to rodent coronaviruses
- PMID: 3025244
- PMCID: PMC7119637
- DOI: 10.1016/0166-0934(86)90035-2
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Comparative Study
Two enzyme immunoassays for the detection of antibody to rodent coronaviruses
J Virol Methods.
1986 Nov.
Abstract
Two enzyme immunoassays for detection of antibody to rodent coronaviruses were compared. Mouse hepatitis virus (MHV), strain JHM, antigen was in the form of formalin-fixed, infected 17 C1 1 cells. This antigen detected antibody to the homologous strain of MHV as well as to two heterologous MHV strains and a serologically related rat coronavirus, sialodacryodenititis virus. Antibody titers in assays using horseradish peroxidase (HRP)-conjugated or ureiase-conjugated anti-mouse IgG were substantially higher than in an indirect immunofluorescence assay. The ureiase assay was somewhat more sensitive than the HRP assay. MHV-JHM antigen was stable under a variety of storage conditions for at least two months.
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