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Review
. 2018;6(3):1479568.
doi: 10.1080/21688370.2018.1479568. Epub 2018 Sep 25.

Cell culture models of oral mucosal barriers: A review with a focus on applications, culture conditions and barrier properties

Lisa Bierbaumer  1 , Uwe Yacine Schwarze  2   3 , Reinhard Gruber  2   3   4 , Winfried Neuhaus  1
Affiliations
Review

Cell culture models of oral mucosal barriers: A review with a focus on applications, culture conditions and barrier properties

Lisa Bierbaumer et al. Tissue Barriers. 2018.

Abstract

Understanding the function of oral mucosal epithelial barriers is essential for a plethora of research fields such as tumor biology, inflammation and infection diseases, microbiomics, pharmacology, drug delivery, dental and biomarker research. The barrier properties are comprised by a physical, a transport and a metabolic barrier, and all these barrier components play pivotal roles in the communication between saliva and blood. The sum of all epithelia of the oral cavity and salivary glands is defined as the blood-saliva barrier. The functionality of the barrier is regulated by its microenvironment and often altered during diseases. A huge array of cell culture models have been developed to mimic specific parts of the blood-saliva barrier, but no ultimate standard in vitro models have been established. This review provides a comprehensive overview about developed in vitro models of oral mucosal barriers, their applications, various cultivation protocols and corresponding barrier properties.

Keywords: ABC-transporters; SLC-transporter; craniomaxillofacial surgery; desmosomes; gingivitis; periodontitis; saliva; salivary gland; tight junctions; tongue.

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References

    1. Ganz T. Epithelia: not just physical barriers. Proc Natl Acad Sci U S A. 2002;99(6):3357–8. doi:10.1073/pnas.072073199. - DOI - PMC - PubMed
    1. Richter JF, et al. A novel method for imaging sites of paracellular passage of macromolecules in epithelial sheets. J Control Release 2016;229:70–9. doi:10.1016/j.jconrel.2016.03.018. - DOI - PubMed
    1. Presland RB, Jurevic RJ.. Making sense of the epithelial barrier: what molecular biology and genetics tell us about the functions of oral mucosal and epidermal tissues. J Dent Educ. 2002;66(4):564–74. - PubMed
    1. Li Q, et al. Interferon-gamma and tumor necrosis factor-alpha disrupt epithelial barrier function by altering lipid composition in membrane microdomains of tight junction. Clin Immunol. 2008;126(1):67–80. doi:10.1016/j.clim.2007.08.017. - DOI - PubMed
    1. Ye D, Ma I, Ma TY.. Molecular mechanism of tumor necrosis factor-alpha modulation of intestinal epithelial tight junction barrier. Am J Physiol Gastrointest Liver Physiol. 2006;290(3):G496–504. doi:10.1152/ajpgi.00318.2005. - DOI - PubMed

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