Effect of mitochondrial cytochromes and haem content on cytochrome P450 in Saccharomyces cerevisiae

Abstract

It is well established that the mitochondrial and the microsomal cytochromes in Saccharomyces cerevisiae are regulated differently. Mutations affecting the mitochondrial cytochromes aa3 or c had no effect on the concentration of the microsomal cytochrome P450 even during haem limitation. Moreover, a defect in the cytochrome P450 gene did not affect mitochondrial cytochromes. However, a regulatory mutation present in strain SG1 decreased both mitochondrial and microsomal cytochrome contents. This mutation also affected the intracellular haem concentration. The haem precursor 5-aminolaevulinate increased both mitochondrial and microsomal cytochrome contents. Our results indicate that carbon source and haem concentration are involved in the regulation of cytochrome P450.