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. 2018 Sep 4:2018:2813465.
doi: 10.1155/2018/2813465. eCollection 2018.

Lepidiumuridine A: A New Natural Uridine Derivative as a Phytoestrogen Isolated from the Seeds of Lepidium apetalum Willd

Meng Li #  1   2 , Mengnan Zeng #  1   2 , Zhi-Guang Zhang  1   2 , Beibei Zhang  1   2 , Jingke Zhang  1   2 , Xiaoke Zheng  1   2 , Weisheng Feng  1   2
Affiliations

Lepidiumuridine A: A New Natural Uridine Derivative as a Phytoestrogen Isolated from the Seeds of Lepidium apetalum Willd

Meng Li et al. Evid Based Complement Alternat Med. .

Abstract

There has been great interest in phytoestrogens, which are polyhydric compounds that are derived from plants and have a structure similar to that of the mammalian steroid hormone 17β-estradiol. The present study examined the estrogenic effects of a new natural uridine derivative, lepidiumuridine A (LA), that was isolated from the seeds of Lepidium apetalum. The structure was clarified and determined via analysis of extensive spectroscopic data interpretation. The activity of LA was investigated by measuring the levels of estradiol (E2), luteinizing hormone (LH), follicle stimulating hormone (FSH), and the uterus growth in mice. The proliferation experiment of MCF-7 breast cancer cells was also conducted. Western blot, in-cell western, and antagonist assays with methyl piperidino-pyrazole (MPP) were used for exploring the mechanism of the effects of LA. The results showed that LA elevated the uterine coefficient, the levels of E2, and FSH significantly. In addition, LA significantly elevated ERα expression in the uterus and MCF-7 cells. MPP inhibited the proliferation of LA-stimulated MCF-7 cell and ERα expression in MCF-7 cells. Taken together, LA had an estrogen-like effect, which was mainly mediated by the estrogen receptor ERα.

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Figures

Figure 1
Figure 1
Structure and selected HMBC correlations of compound 1.
Figure 2
Figure 2
Effect of LA on the uterine expression of ERα, ERβ and GPR30 (n = 3). Low, High: low and high dose of LA. ∗∗P< 0.01 compared to the control group.
Figure 3
Figure 3
The influence of MCF-7 cell proliferation. Data represent the mean ± SD, n=4; ∗P< 0.05; ∗∗P< 0.01.
Figure 4
Figure 4
Effect of MPP on MCF-7 cell proliferation. The MPP were appended 0.5h before treatment of 17β-E2 and LA. Data represent the mean ± SD, n=3; ∗∗P< 0.01.
Figure 5
Figure 5
In-cell western tested the expression of ERα in MCF-7 cells. Data represent the mean ± SD, n=4; ∗P< 0.05; ∗∗P< 0.01.
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