Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Apr 13;6(1):48-52.
doi: 10.1016/j.ijvsm.2018.04.005. eCollection 2018 Jun.

Phylogeny of bovine norovirus in Egypt based on VP2 gene

Fakry F Mohamed  1   2 , Gamelat K F Ktob  1 , Mohamed E A Ismaeil  1 , Ahmed A H Ali  1 , Sagar M Goyal  2
Affiliations

Phylogeny of bovine norovirus in Egypt based on VP2 gene

Fakry F Mohamed et al. Int J Vet Sci Med. .

Abstract

Bovine norovirus (BNoV) has emerged as a viral pathogen that causes a gastrointestinal illness and diarrhea in cattle. Despite its worldwide distribution, very little information is known about BNoV in Africa. In this study, BNoV was detected in 27.6% (8/29) of tested fecal materials, collected from sporadic cases of diarrheic calves, using the reverse transcription-polymerase chain reaction (RT-PCR) and primers that target RNA dependent RNA polymerase gene. Additionally, one primer pair was designed to flank the BNoV-VP2 (small capsid protein) gene for molecular analysis. Study VP2 sequences were phylogenetically-related to BNoV-GIII.2 (Newbury2-like) genotype, which is highly prevalent all over the world. However, they were separated within the cluster and one strain (41FR) grouped with recombinant GIII.P1/GIII.2 strains. Compared to reference VP2 sequences, 14 amino acid substitution mutations were found to be unique to our strains. The study confirms that BNoV is currently circulating among diarrheic calves of Egypt and also characterizes its ORF3 (VP2) genetically. The status of BNoV should be continuously evaluated in Egypt for effective prevention and control.

Keywords: Caliciviruses; Diarrhea; Gastroenteritis; Genogroups; ORF3; Phylogeny.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
The RT-PCR amplification of BNoV-RdRp genes from study fecal RNA samples. Lane M; DNA marker (50–10.000 bp). Lane 12 (Ctrl-); negative control. Lanes 2, 5, 7, and 11; BNoV positive samples (532 bp).
Fig. 2
Fig. 2
The neighbor-joining phylogenetic tree of BNoV-VP2 nt sequences. Bootstrap values of >50% are shown above the branches. The study strains (41FR and 42FR) are indicated with black circles. Strains of Nebovirus genus were chosen as an outgroup.
Fig. 3
Fig. 3
Residue substitutions in deduced amino acids (aa) of BNoV-GIII.2 strains compared to the consensus sequence. Majority is the consensus represented on the ruler. Points mean identical aa while dashes mean unresolved aa. Unique aa substitutions in the study strains are marked with red arrows.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Scipioni A., Mauroy A., Vinje J., Thiry E. Animal noroviruses. Vet J. 2008;178:32–45. - PubMed
    1. Van der Poel W.H., van der Heide R., Verschoor F., Gelderblom H., Vinjé J., Koopmans M.P. Epidemiology of Norwalk-like virus infections in cattle in The Netherlands. Vet Microbiol. 2003;92:297–309. - PubMed
    1. Jor E., Myrmel M., Jonassen C.M. SYBR Green based real-time RT-PCR assay for detection and genotype prediction of bovine noroviruses and assessment of clinical significance in Norway. J Virol Methods. 2010;169:1–7. - PMC - PubMed
    1. Di Martino B., Di Profio F., Di Felice E., Melegari I., Ceci C., Mauroy A. Genetic heterogeneity of bovine noroviruses in Italy. Arch Virol. 2014;159:2717–2722. - PubMed
    1. Otto P.H., Clarke I.N., Lambden P.R., Salim O., Reetz J., Liebler-Tenorio E.M. Infection of calves with bovine norovirus GIII. 1 strain Jena virus: an experimental model to study the pathogenesis of norovirus infection. J Virol. 2011;85:12013–12021. - PMC - PubMed

LinkOut - more resources