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. 2018 Sep 25;12(1):100.
doi: 10.1186/s13065-018-0468-4.

Physicochemical properties of polysaccharides from Dendrobium officinale by fractional precipitation and their preliminary antioxidant and anti-HepG2 cells activities in vitro

Shangping Xing  1 , Xiaofeng Zhang  1 , Hannu Ke  1 , Ji Lin  1 , Yuechun Huang  2 , Gang Wei  3
Affiliations

Physicochemical properties of polysaccharides from Dendrobium officinale by fractional precipitation and their preliminary antioxidant and anti-HepG2 cells activities in vitro

Shangping Xing et al. Chem Cent J. .

Erratum in

Abstract

Background: Dendrobium officinale as a precious traditional Chinese herb is widely used in medicines and health supplements. Thus the extraction, purification and biological activities of polysaccharides from the stem of Dendrobium officinale have significant meaning on theory and application value.

Methods: The crude Dendrobium officinale polysaccharide (DOP) was obtained by hot water extraction- ethanol precipitation method, and four new polysaccharide fractions (DOP-40, DOP-50, DOP-60, and DOP-70) were further obtained from the crude DOP by fractional precipitation with ethanol method, then four fractions were further purified by Toyopearl-H65F gel resin. The molecular weight and monosaccharide composition of four purified fractions were determined by high performance anion exchange chromatography and high performance liquid chromatography. The antioxidant activities of them were evaluated by the reducing power assay, and the superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and hydroxyl free radicals scavenging assays, respectively. Finally, the anticancer activities of them were investigated via the MTT assay and the western blot analysis using HepG2 cells.

Results: Among these four purified fractions were mainly composed of D-mannose and D-glucose with different molar ratios, and their average molecular weights were 999, 657, 243 and 50.3 kDa, respectively. What's more, DOP-70 always exhibited the strongest antioxidant and anticancer activities, while DOP-40 and DOP-60 showed very close antioxidant and anticancer activities which were better than that of DOP-50. The western blotting analysis also showed that DOP-40, DOP-60, and DOP-70 induced apoptosis in HepG2 human liver cancer cells through the Bcl-2 and Bax-dependent pathway.

Conclusions: Fractional precipitation with ethanol could successfully apply to extract four new polysaccharide fractions from Dendrobium officinale stems, and the polysaccharide fractions possessed efficient antioxidant and anticancer activities, especially DOP-70.

Keywords: Antioxidant activity; Dendrobium officinale; Fractional precipitation; HepG2 cells; Polysaccharide fractions.

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Conflict of interest statement

All authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Images of Dendrobium officinale (a), and its stems (b)
Fig. 2
Fig. 2
Extraction and fractionation procedure of polysaccharide fractions from Dendrobium officinale stems
Fig. 3
Fig. 3
Distilled NaCl elution curves of DOP-40 (a), DOP-50 (b), DOP-60 (c), and DOP-70 (d)
Fig. 4
Fig. 4
HPLC profile of mixed monosaccharide standards (1-mannose, 2-rhamnose, 3-galactose, 4-glucose, and 5-arabinose), DOP-40, DOP-50, DOP-60, and DOP-70
Fig. 5
Fig. 5
Scavenging activities on the reducing power assay (a), superoxide anion radical (b), DPPH radical (c), and hydroxyl radical (d) for DOP-40, DOP-50, DOP-60, and DOP-70
Fig. 6
Fig. 6
Growth inhibition analysis of HepG2 cells treated with DOP-40, DOP-50, DOP-60, and DOP-70 for 24 h (a) and 48 h (b) by MTT assay. The protein levels of Bcl-2 (c, e) and Bax (c, f) in HepG2 cells treated with DOP-40, DOP-50, DOP-60, and DOP-70 (400 μg/ml) for 48 h were analyzed by western blotting. The bar graphs (mean ± SD) and representative images are shown. *p < 0.05; **p < 0.01 compared with the control group
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References

    1. Ding X, Wang Z, Zhou K, Xu L, Xu H, Wang Y. Allele-specific primers for diagnostic PCR authentication of Dendrobium officinale. Planta Med. 2003;69:587–588. doi: 10.1055/s-2003-40651. - DOI - PubMed
    1. Hou B, Luo J, Zhang Y, Niu Z, Xue Q, Ding X. Iteration expansion and regional evolution: phylogeography of Dendrobium officinale and four related taxa in southern China. Sci Rep. 2017;7:43525. doi: 10.1038/srep43525. - DOI - PMC - PubMed
    1. Liu XF, Zhu J, Ge SY, Xia LJ, Yang HY, Qian YT, Ren FZ. Orally administered Dendrobium officinale and its polysaccharides enhance immune functions in BALB/c mice. Nat Prod Commun. 2011;6:867–870. - PubMed
    1. Luo QL, Tang ZH, Zhang XF, Zhong YH, Yao SZ, Wang LS, Lin CW, Luo X. Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale. Int J Biol Macromol. 2016;89:219–227. doi: 10.1016/j.ijbiomac.2016.04.067. - DOI - PubMed
    1. Zhao Y, Liu Y, Lan XM, Xu GL, Sun YZ, Li F, Liu HN. Effect of Dendrobium officinale extraction on gastric carcinogenesis in rats. Evid Based Complement Alternat Med. 2016;2016:1213090. - PMC - PubMed

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