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. 1986 May:(5):24-6.

[New specific endonucleases PaeI and PaeII from Pseudomonas aeruginosa]

[Article in Russian]
N N Sokolov, V O Kolosha, A B Fitsner, N V Anikeĭcheva, E B Khoroshutina
  • PMID: 3025709

[New specific endonucleases PaeI and PaeII from Pseudomonas aeruginosa]

[Article in Russian]
N N Sokolov et al. Mol Gen Mikrobiol Virusol. 1986 May.

Abstract

Specific endonuclease activities have been found it two Pseudomonas aeruginosa strains. Isolation and purification of enzymes and determining their specific activities have permitted one to find out that PaeI is an isoshizomer of SphI and digests the sequence 5'-GCATG C-3'. Another isolated enzyme PaeII is an isoshizomer of SmaI and cleaves DNA in a fragment 5'-CCC GGG-3'. The use of PaeI and PaeII enzymes in genetical engineering and their advantages are discussed.

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