[New specific endonucleases PaeI and PaeII from Pseudomonas aeruginosa]
[Article in
Russian]
- PMID: 3025709
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[New specific endonucleases PaeI and PaeII from Pseudomonas aeruginosa]
[Article in
Russian]
Mol Gen Mikrobiol Virusol.
1986 May.
Abstract
Specific endonuclease activities have been found it two Pseudomonas aeruginosa strains. Isolation and purification of enzymes and determining their specific activities have permitted one to find out that PaeI is an isoshizomer of SphI and digests the sequence 5'-GCATG C-3'. Another isolated enzyme PaeII is an isoshizomer of SmaI and cleaves DNA in a fragment 5'-CCC GGG-3'. The use of PaeI and PaeII enzymes in genetical engineering and their advantages are discussed.
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