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. 2018 Sep 26;14(1):295.
doi: 10.1186/s12917-018-1622-x.

Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease

Kun Qian  1   2   3 , Xue Tian  1 , Hongxia Shao  1   2   3 , Jianqiang Ye  1   2   3 , Yongxiu Yao  4   5 , Venugopal Nair  4   5 , Aijian Qin  6   7   8
Affiliations

Identification of novel B-cell epitope in gp85 of subgroup J avian leukosis virus and its application in diagnosis of disease

Kun Qian et al. BMC Vet Res. .

Abstract

Background: The gp85 is the main envelope protein of avian leukosis subgroup J (ALV-J) involved in virus neutralization. Here, we mapped the epitope in ALV-J gp85 by ELISA using synthetic peptides and developed epitope based diagnostic methods for ALV-J infection.

Results: The results revealed that monoclonal antibody (mAb) JE9 recognized 83WDPQEL88 motif, which was highly conserved in gp85 among different ALV-J strains by homology analysis. Moreover, after evaluation with two hundred and forty sera samples obtained from different chicken farms, the epitope-based peptide ELISA had much higher sensitivity than commercial ELISA kit for antibody detection of ALV-J.

Conclusions: A novel B-cell epitope recognized by the mAb JE9 was identified. The developed peptide-ELISA based on this novel B-cell epitope could be useful in laboratory viral diagnosis, routine surveillance in chicken farms, and also in understanding the pathogenesis of ALV-J.

Keywords: Antibody detection; Avian leukosis virus subgroup J; B-cell epitope mapping; Epitope-based peptide ELISA.

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Conflict of interest statement

Ethics approval and consent to participate

The 170 SPF chicken sera samples were kindly provided by Spirax Ferrer Poultry Science and Technology Co.Ltd., Jinan, China. The other 240 chicken sera samples were collected from chicken farms with the eradication programme of ALV being conducted in Jiangsu and Shandong Province in China. All experiments complied with institutional animal care guidelines and were approved by the Animal Care Committee of Yangzhou University.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Reactivity of the different synthetic peptides of gp85 with mAb JE9 using ELISA. The name of each column corresponds to the polypeptide in the Table 2
Fig. 2
Fig. 2
Alignment of the epitopes motif with 51 ALV strains. The GenBank accession numbers of the ALV strains used are indicated in parentheses. The homologous sequences of different ALV strains corresponding to the identified epitope are boxed. Identical residues are indicated by “.”. “-” indicates that there was no corresponding amino acid at this position
Fig. 3
Fig. 3
The result of partial serum samples evaluated with IFA. Panel a and b were negative and positive control respectively; Panel c to f were partial positive results by serum samples which were negative in IDEXX ELISA, but positive in peptide-ELISA
See this image and copyright information in PMC

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