Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Dec;83(24):9303-7.
doi: 10.1073/pnas.83.24.9303.

Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae

P Sass, J Field, J Nikawa, T Toda, M Wigler

Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae

P Sass et al. Proc Natl Acad Sci U S A. 1986 Dec.

Abstract

A gene, PDE2, has been cloned from the yeast Saccharomyces cerevisiae that, when present in high copy, reverses the phenotypic effects of RAS2Val19, a mutant form of the RAS2 gene that renders yeast cells sensitive to heat shock and starvation. It has previously been shown that the RAS proteins are potent activators of yeast adenylate cyclase. We report here that PDE2 encodes a high-affinity cAMP phosphodiesterase that shares sequence homology with animal cell phosphodiesterases. These results therefore imply that the effects of RAS2Val19 are mediated through its changes in cAMP concentration.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Biochemistry. 1971 Jan 19;10(2):311-6 - PubMed
    1. Gene. 1985;33(1):103-19 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. J Biol Chem. 1978 Apr 25;253(8):2518-21 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 - PubMed

Publication types

Associated data