Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Jan;84(1):214-8.
doi: 10.1073/pnas.84.1.214.

Chimeric antibody with human constant regions and mouse variable regions directed against carcinoma-associated antigen 17-1A

L K Sun, P Curtis, E Rakowicz-Szulczynska, J Ghrayeb, N Chang, S L Morrison, H Koprowski

Chimeric antibody with human constant regions and mouse variable regions directed against carcinoma-associated antigen 17-1A

L K Sun et al. Proc Natl Acad Sci U S A. 1987 Jan.

Abstract

We have cloned the genomic DNA fragments encoding the heavy and light chain variable regions of monoclonal antibody 17-1A, and we have inserted them into mammalian expression vectors containing genomic DNA segments encoding human gamma 3 and kappa constant regions. The transfer of these expression vectors containing mouse-human chimeric immunoglobulin genes into Sp2/0 mouse myeloma cells resulted in the production of functional IgG that retained the specific binding to the surface antigen 17-1A expressed on colorectal carcinoma cells.

PubMed Disclaimer

References

    1. J Immunol. 1985 Aug;135(2):1530-5 - PubMed
    1. Nature. 1985 Apr 4-10;314(6010):452-4 - PubMed
    1. Cancer Res. 1985 Nov;45(11 Pt 2):5910-3 - PubMed
    1. J Immunol. 1986 Feb 15;136(4):1497-503 - PubMed
    1. Nature. 1984 Dec 13-19;312(5995):604-8 - PubMed

Associated data

LinkOut - more resources