Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Jul 20;6(6):1676-1683.
doi: 10.1002/fsn3.741. eCollection 2018 Sep.

Antioxidant and alpha-amylase inhibitory potentials of Cocos nucifera husk

Hamdalat Folake Muritala  1 , Jubril Olayinka Akolade  2 , Sarah Abimbola Akande  2 , Azeemat Titilola Abdulazeez  3 , Raliat Abimbola Aladodo  4 , Abdulkabir Bolakale Bello  1
Affiliations

Antioxidant and alpha-amylase inhibitory potentials of Cocos nucifera husk

Hamdalat Folake Muritala et al. Food Sci Nutr. .

Abstract

Concoctions containing extract from Cocos nucifera husk fiber are used in Nigeria by traditional medicine practitioners for management of diabetes and its associated complications. Preliminary antidiabetic study was designed to validate the folkloric usage of the plant extract. Dried coconut husk fiber was pulverized and extracted with methanol, followed by partitioning of the methanolic extract in ethyl acetate. Phenolic content, radical scavenging activity and antioxidant capacity as well as inhibitory effects of C. nucifera methanolic (CN-M) extract and its ethyl acetate (CN-E) fraction on pancreatic α-amylase and lipid peroxidation were determined. Total phenolic content and antioxidant capacity of CN-E fraction were significantly higher than that of CN-M extract, whereas there was no significant difference in their ability to scavenge free radicals. The CN-E fraction also exhibited higher in vitro and in vivo inhibitory effects on α-amylase activity and lipid peroxidation; reducing blood glucose level within 5 days following intraperitoneal administration of the C. nucifera extract to alloxan-induced hyperglycemic rats. The phenolic-rich extracts from coconut husk can be further explored as nutraceutical supplement in food formulation for diabetic patients.

Keywords: Cocus nucifera; alpha‐amylase; antioxidants; diabetes mellitus; functional foods.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Schematic representation for preparation of Cocos nucifera methanolic extract (CN‐M) and its ethyl acetate fraction (CN‐E)
Figure 2
Figure 2
Total pocolyphenolic content of Cocos nucifera husk methanolic extract (CN‐M) and its ethyl acetate fraction (CN‐E) *GA: Gallic Acid
Figure 3
Figure 3
Total antioxidant capacity Cocos nucifera methanolic extract (CN‐M) and its ethyl acetate fraction (CN‐E) *GA: Gallic Acid. *Significantly higher at p < 0.05
Figure 4
Figure 4
Radical scavenging activity of Cocos nucifera methanolic extract (CN‐M) and its Ethyl acetate fraction (CN‐E). *GA: Gallic Acid
Figure 5
Figure 5
Lipid peroxidation (LP) inhibitory effect of Cocos nucifera methanolic extract (CN‐M) and its ethyl acetate fraction (CN‐E)
Figure 6
Figure 6
Alpha‐amylase inhibitory activity of Cocos nucifera methanolic extract (CN‐M) and its ethyl acetate fraction (CN‐E). *ACA: Acarbose
Figure 7
Figure 7
Concentration of reduced glutathione in the pancreas of alloxan‐induced diabetic rats treated with Cocos nucifera methanolic extract (CN‐M) and its ethyl acetate fraction (CN‐E). *NIC: Noninduced control; AIC: Alloxan‐induced control; MET: Metformin
Figure 8
Figure 8
Specific activity of pancreatic alpha‐amylase in alloxan‐induced diabetic rats treated with Cocos nucifera methanolic extract (CN‐M) and its ethyl acetate fraction (CN‐E). *NIC: Noninduced control; AIC: Alloxan‐induced control; MET: Metformin
Figure 9
Figure 9
Concentration of malondialdehyde in the pancreas of alloxan‐induced diabetic rats treated with Cocos nucifera methanolic extract (CN‐M) and its ethyl acetate fraction (CN‐E) *NIC: Noninduced control; AIC: Alloxan‐induced control; MET: Metformin
See this image and copyright information in PMC

References

    1. Adaramoye, O. A. , Azeez, A. F. , & Ola‐Davies, O. E. (2016). Ameliorative effects of chloroform fraction of Cocos nucifera L. husk fiber against Cisplatin‐induced toxicity in rats. Pharmacognosy Research, 8(2), 89–96. 10.4103/0974-8490.172658 - DOI - PMC - PubMed
    1. Akolade, J. O. , Usman, L. A. , Okereke, O. E. , & Muhammad, N. O. (2014). Antidiabetic potentials of essential oil extracted from the leaves of Hoslundia opposita Vahl. Journal of Medicinal Food, 17(10), 1122–1128. 10.1089/jmf.2013.0118 - DOI - PubMed
    1. Alam, M. N. , Bristi, N. J. , & Rafiquzzaman, M. (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21(2), 143–152. 10.1016/j.jsps.2012.05.002 - DOI - PMC - PubMed
    1. American Diabetes Association . (2014). Diagnosis and classification of diabetes mellitus. Diabetes Care, 37(Suppl 1), S81–S90. 10.2337/dc14-S081 - DOI - PubMed
    1. Bezerra dos Santos Oliveira, M. , Barros Valentim, I. , Calado de Vasconcelos, C. , Maria Bazilio Omena, C. , Jose Henriques Bechara, E. , Gomes da Costa, J. , … Oliveira Fonseca Goulart, M. (2013). Cocos nucifera Linn.(Palmae) husk fiber ethanolic extract: Antioxidant capacity and electrochemical investigation. Combinatorial Chemistry & High Throughput Screening, 16(2), 121–129. - PubMed

LinkOut - more resources