[In vitro transcription of human influenza and parainfluenza viruses and its regulation]

Abstract

The effect of some factors on in vitro transcription of human influenza and parainfluenza viruses was studied. Dinucleotide AfG was shown to stimulate transcription of RNP of human parainfluenza type 3 virus and Sendai virus in the presence of magnesium but not manganese ions same as in the case of influenza viruses transcription. Among two monoclonal antibodies to NP protein of influenza virus, clone F 81 inhibited transcription of RNP of human influenza A viruses, and its influence on transcription of animal influenza viruses was weak. Another clone, H 12, had a low effect. The M protein isolated from influenza and parainfluenza virions inhibited in vitro transcription in the corresponding homologous systems. The inhibiting effect was exerted also by the M protein heterologous to HPIV-3 (M protein of Sendai virus) which suggests the nonspecificity of M protein interaction with transcriptive complexes in large RNA viruses.