Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Feb 29;25(1):193-198.
doi: 10.1007/s10068-016-0029-3. eCollection 2016.

Detection of Campylobacter DNA using magnetic nanoparticles coupled with PCR and a colorimetric end-point system

Wuttichote Jansaento  1 , Kulachart Jangpatarapongsa  2 , Duangporn Polpanich  3 , Wijit Wonglumsom  1
Affiliations

Detection of Campylobacter DNA using magnetic nanoparticles coupled with PCR and a colorimetric end-point system

Wuttichote Jansaento et al. Food Sci Biotechnol. .

Abstract

Campylobacter is an important food-borne pathogen causing acute gastroenteritis worldwide. Magnetic nanoparticle-based PCR coupled with streptavidin-horseradish peroxidase and a substrate was used for colorimetric detection. Forward primers conjugated to magnetic nanoparticles facilitated separation and concentration of Campylobacter DNA in a sample matrix. After PCR, a green color developed and was observed using the unaided eye, or detected using a spectrophotometer. High specificity and sensitivity of the 100 fg DNA/PCR reaction were achieved in pure culture experiments. The technique was applied for detection of Campylobacter on naturally contaminated chicken skin. All positive results were in agreement with results achieved using a conventional culture method. The magnetic nanoparticle-PCR-enzyme linked gene assay was practical and useful for detection of Campylobacter in complex matrices with PCR-interfering substances.

Keywords: Campylobacter; chicken; colorimetric system; magnetic nanoparticle.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Butzler JP. Campylobacter from obscurity to celebrity. Clin. Microbiol. Infec. 2004;10:868–876. doi: 10.1111/j.1469-0691.2004.00983.x. - DOI - PubMed
    1. Hughes RA, Rees JH. Clinical and epidemiologic features of Guillain-Barré syndrome. J. Infect. Dis. 1997;176:92–98. doi: 10.1086/513793. - DOI - PubMed
    1. Hannu T, Mattlia L, Rautelin H, Pelkonen P, Lahdenne P, Siitonen A, Leirisalo-Repo M. Campylobacter-triggered reactive arthritis: A population-based study. Rheumatolog. 2002;41:312–318. doi: 10.1093/rheumatology/41.3.312. - DOI - PubMed
    1. Young KT, Davis LM, Dirita VJ. Campylobacter jejuni: Molecular biology and pathogenesis. Nat. Rev. Microbiol. 2007;5:665–679. doi: 10.1038/nrmicro1718. - DOI - PubMed
    1. Vindigni SM, Srijan A, Wongstitwilairoong B, Marcus R, Meek J, Riley PL, Mason C. Prevalence of foodborne microorganisms in retail foods in Thailand. Foodborne Pathog. Dis. 2007;4:208–215. doi: 10.1089/fpd.2006.0077. - DOI - PubMed

LinkOut - more resources