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. 1986 Sep 1;238(2):517-21.
doi: 10.1042/bj2380517.

The radiosensitivity of rat thymocytes

M G Ord, L A Stocken

The radiosensitivity of rat thymocytes

M G Ord et al. Biochem J. .

Abstract

gamma-Irradiation in vitro apparently blocked a plasma-membrane associated, superoxide-producing, NADPH oxidase in rat thymocytes. Differential centrifugation of the mixed thymocytes indicated the smaller lymphocytes (approx. 6 microns diameter) to be the radiosensitive population. The oxidase system co-isolated in part with thymus nuclei and could be solubilized by detergent treatment [Bellavite, Jones, Cross, Papini & Rossi (1984) Biochem. J. 223, 639-648]. Endogenous NADPH was the rate-limiting component for superoxide formation in vitro. The level of NADPH was lowered by gamma-irradiation, an effect mimicked by GSSG in the presence of 50 microM-ZnCl2 to inhibit GSSG reductase. These findings are suggested as the metabolic basis for interphase death of small lymphocytes exposed to ionizing radiation.

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References

    1. Nature. 1963 Oct 12;200:136-8 - PubMed
    1. Biochem J. 1984 Sep 1;222(2):541-51 - PubMed
    1. Int J Cancer. 1976 May 15;17(5):565-77 - PubMed
    1. Thymus. 1984;6(4):235-42 - PubMed
    1. Am J Pathol. 1982 Jun;107(3):395-418 - PubMed