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. 2017 May 29;26(3):759-766.
doi: 10.1007/s10068-017-0098-y. eCollection 2017.

Different cell disruption methods for obtaining carotenoids by Sporodiobolus pararoseus and Rhodothorula mucilaginosa

Nathalie Almeida Lopes  1 , Rafael Diaz Remedi  1 , Carolina Dos Santos Sá  1 , Carlos André Veiga Burkert  1 , Janaina Fernandes de Medeiros Burkert  1
Affiliations

Different cell disruption methods for obtaining carotenoids by Sporodiobolus pararoseus and Rhodothorula mucilaginosa

Nathalie Almeida Lopes et al. Food Sci Biotechnol. .

Abstract

Since carotenoids are synthesized inside the cell, it is desirable to find an efficient method to extract carotegenic pigments. This study aimed at comparing the effectiveness of different chemical and mechanical techniques to disrupt the cell wall of Sporidiobolus pararoseus and Rhodotorula mucilaginosa yeasts isolated from environmental samples. Among the techniques under study, the ultrasonic bath and the abrasion with glass beads yielded the most promising results for S. pararoseus (84.8 ± 2.3 and 76.9 ± 2.1 μg/g, respectively). The ultrasonic bath yielded the highest specific concentration of carotenoids for R. mucilaginosa (193.5 ± 25.8 μg/g), while the biomass freezing process improved neither the extractability nor the specific concentration of carotenoids. Lyophilization increased the specific concentrations of carotenoids from S. pararoseus and R. mucilaginosa by 20 and 13.7%, respectively, while the freezing process did not significantly affect (p > 0.05) the recovery of carotenoids from both yeasts; thus, it may be eliminated from the process.

Keywords: Biomass; Carotenoids; Freezing; Ultrasonic bath; Yeast.

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Conflict of interest statement

Compliance with ethical standardsThe authors declare no conflicts of interest.

Figures

Fig. 1
Fig. 1
Yeast cells S. pararoseus (A) and R. mucilaginosa (C) without disruption; cells of S. pararoseus (B) and R. mucilaginosa (D) after disruption via ultrasonic bath
Fig. 2
Fig. 2
Concentrations of total carotenoids (µg/g) in treatment 1: Lyophilization (frozen biomass); treatment 2: Lyophilization (not frozen biomass); treatment 3: conventional drying (frozen biomass); treatment 4: Conventional drying (not frozen biomass). Different letters represent significant differences among the columns for the same microorganism (p < 0.05)
See this image and copyright information in PMC

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