Antioxidant and hepatoprotective activity of kaempferol 3- O-β-d- (2,6-di- O-α-l-rhamnopyranosyl)galactopyronoside against carbon tetrachloride-induced liver injury in mice

Abstract

This study aims to investigate the antioxidant and hepatoprotective effects of kaempferol 3-O-β-d- (2,6-di-O-α-l-rhamnopyranosyl)galactopyronoside (KG) isolated from unripe soybean leaves. Carbon tetrachloride (CCl₄)-induced hepatotoxic ddY mice were used in the study. The mice were divided into three groups, namely the control group, the CCl₄ group (CCl₄, CCl₄ injected), and the KG group (KG, CCl₄ injected with KG administration). Hepatic injury markers of serum and liver were analyzed. The results show that serum ALT, AST activities, hepatic glutathione, superoxide dismutase, catalase, and glutathione peroxidase activities were normalized in mice pretreated with KG. Furthermore, the liver thiobarbituric acid reactive substances levels were found to be improved by pretreatment with KG, indicating that KG is available to alleviate liver injury, this may be due to its antioxidant properties. This study suggests that unripe soy leaves could be used as functional food materials.

Keywords: Antioxidant activity; CCl4; Hepatotoxicity; Kaempferol 3-O-β-d- (2,6-di-O-α-l-rhamnopyranosyl)galactopyronoside; Mice.

Fig. 1

HPLC chromatograms and chemical structure of kaempferol 3-O-β-d- (2,6-di-O-α-l-rhamnopyranosyl)galactopyronoside (KG) isolated from unripe soybean (Jindai) leaves

Fig. 2

Effects of KG on serum ALT (A) and AST (B) activities. Each value is mean ± SEM; n = 7 for each group. Values without a common letter differ significantly (p < 0.05). CON: control-group mice; CCl₄: CCl₄-injected-group mice; KG: CCl₄-injected-group mice with KG administration

Fig. 3

Effects of KG on liver GSH (A) and GSSG (B) levels. Each value is mean ± SEM; n = 7 for each group. Values without a common letter differ significantly (p < 0.05). CON: control-group mice; CCl₄: CCl₄-injected-group mice; KG: CCl₄-injected-group mice with KG administration

Fig. 4

Effects of KG on liver SOD (A), GSH-Px (B) and CAT (C) activities. Each value is mean ± SEM; n = 7 for each group. Values without a common letter differ significantly (p < 0.05). CON: control-group mice; CCl₄: CCl₄-injected-group mice; KG: CCl₄-injected-group mice with KG administration

Fig. 5

Effect of KG on liver TBARS level. Each value is mean ± SEM; n = 7 for each group. Values without a common letter differ significantly (p < 0.05). CON: control-group mice; CCl₄: CCl₄-injected-group mice; KG: CCl₄-injected-group mice with KG administration