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. 2018 Oct 25;54(83):11701-11704.
doi: 10.1039/c8cc06966b. Epub 2018 Sep 28.

Unraveling the isomeric heterogeneity of glycans: ion mobility separations in structures for lossless ion manipulations

Gabe Nagy  1 , Isaac K Attah, Sandilya V B Garimella, Keqi Tang, Yehia M Ibrahim, Erin S Baker, Richard D Smith
Affiliations

Unraveling the isomeric heterogeneity of glycans: ion mobility separations in structures for lossless ion manipulations

Gabe Nagy et al. Chem Commun (Camb). .

Abstract

To address the challenges associated with glycan analyses, we have implemented a structures for lossless ion manipulations (SLIM) serpentine ultra-long path with extended routing (SUPER) ion mobility-mass spectrometry (i.e. SLIM SUPER IM-MS) platform to achieve much higher resolution of isomeric glycoforms. We have demonstrated the potential of this platform as a future component of the glycomics toolbox.

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Conflict of interest statement

Conflicts of interest

There are no conflicts to declare.

Figures

Figure 1.
Figure 1.
Illustration of some of the isomeric heterogeneity of glycans in various tri/tetra-saccharides (top), and a simplified schematic of the SLIM device ion path used in these experiments (bottom).
Figure 2.
Figure 2.
112.5 m SLIM SUPER IM separation of isomeric disaccharides, 365.1 m/z [M+Na]+.
Figure 3.
Figure 3.
45 m SLIM SUPER IM separation of isomeric trisaccharides, 527.2 m/z (top) and 72 m separation of isomeric tetrasaccharides 689.2 m/z (bottom), both as [M+Na]+. For IM peak assignments, see Supporting Information.
Figure 4.
Figure 4.
31.5 m SLIM SUPER IM separation of human milk oligosaccharide isomers, LNT and LNnT (top) as 708.3 m/z [M+H]+. 45 m separation of lacto-N-fucopentaose isomers (bottom) as 446.6 m/z [M+H+K]2+. Structures are drawn according to conventional glycan symbol nomenclature. For IM peak assignments, see the Supporting Information.
See this image and copyright information in PMC

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