Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2018 Dec:72:99-106.
doi: 10.1016/j.dnarep.2018.09.003. Epub 2018 Sep 12.

Regulation of the initiation of DNA replication in human cells

Tatiana N Moiseeva  1 , Christopher J Bakkenist  2
Affiliations
Review

Regulation of the initiation of DNA replication in human cells

Tatiana N Moiseeva et al. DNA Repair (Amst). 2018 Dec.

Abstract

The origin of species would not have been possible without high fidelity DNA replication and complex genomes evolved with mechanisms that control the initiation of DNA replication at multiple origins on multiple chromosomes such that the genome is duplicated once and only once. The mechanisms that control the assembly and activation of the replicative helicase and the initiation of DNA replication in yeast and Xenopus egg extract systems have been identified and reviewed [1,2]. The goal of this review is to organize currently available data on the mechanisms that control the initiation of DNA replication in human cells.

Keywords: CDC7; CDK2; DDK; Initiation of DNA replication; MCM; Origin firing; Replication checkpoint.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Replication initiation in human cells. 1a. MCM loading; 1b.CMG assembly; 1c. CMG activation. 1d. Full replication complex
Figure 2.
Figure 2.
Replication initiation signaling. 2a. Cdk2 regulation; 2b. Cyclins’ regulation. 2c. Cdc7 regulation. 2d. Dbf4 regulation.
Figure 3.
Figure 3.
Regulation of DNA replication initiation after DNA damage
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Bell SP and Labib K, Chromosome Duplication in Saccharomyces cerevisiae. Genetics, 2016. 203(3): p. 1027–67. - PMC - PubMed
    1. Blow JJ and Laskey RA, Xenopus cell-free extracts and their contribution to the study of DNA replication and other complex biological processes. Int J Dev Biol, 2016. 60(7-8-9): p. 201–207. - PubMed
    1. Jackson DA and Pombo A, Replicon clusters are stable units of chromosome structure: evidence that nuclear organization contributes to the efficient activation and propagation of S phase in human cells. J Cell Biol, 1998. 140(6): p. 1285–95. - PMC - PubMed
    1. Chagin VO, et al., 4D Visualization of replication foci in mammalian cells corresponding to individual replicons. Nat Commun, 2016. 7: p. 11231. - PMC - PubMed
    1. O'Donnell M, Langston L, and Stillman B, Principles and concepts of DNA replication in bacteria, archaea, and eukarya. Cold Spring Harb Perspect Biol, 2013. 5(7). - PMC - PubMed

Publication types

LinkOut - more resources