Insertion element IS1 can generate a 10-base pair target duplication
- PMID: 3026910
- DOI: 10.1016/0378-1119(86)90260-x
Insertion element IS1 can generate a 10-base pair target duplication
Abstract
Transposable element IS1 is known to generate mainly 9-bp and occasionally 8-bp target duplications upon transposition. We have isolated a plasmid pBR322 derivative having IS1 inserted into a site between the promoter and the structural gene for tetracycline resistance. DNA sequence analysis revealed that integration of this IS1 resulted in a 10-bp target duplication.
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