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. 2018 Oct 1.
doi: 10.1038/nbt.4261. Online ahead of print.

Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

Yuan Zong  1   2 , Qianna Song  1   2 , Chao Li  1   2 , Shuai Jin  1   2 , Dingbo Zhang  1   2 , Yanpeng Wang  1 , Jin-Long Qiu  3 , Caixia Gao  1   2
Affiliations

Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

Yuan Zong et al. Nat Biotechnol. .

Erratum in

Abstract

Base editors (BEs) have been used to create C-to-T substitutions in various organisms. However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window and is inefficient in GC contexts. Here, we show that a base editor fusion protein composed of Cas9 nickase and human APOBEC3A (A3A-PBE) converts cytidine to thymidine efficiently in wheat, rice and potato with a 17-nucleotide editing window at all examined sites, independent of sequence context.

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References

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