Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Nov;205(2):326-30.
doi: 10.1007/BF00430446.

Molecular cloning and characterization of ARO7-OSM2, a single yeast gene necessary for chorismate mutase activity and growth in hypertonic medium

S G Ball, R B Wickner, G Cottarel, M Schaus, C Tirtiaux

Molecular cloning and characterization of ARO7-OSM2, a single yeast gene necessary for chorismate mutase activity and growth in hypertonic medium

S G Ball et al. Mol Gen Genet. 1986 Nov.

Abstract

The chorismate mutase structural gene, ARO7, which is necessary for both phenylalanine and tyrosine biosynthesis was cloned by complementation in yeast. Genetic analysis showed that ARO7 was identical to a gene necessary for growth in hypertonic medium, OSM2, which mapped nearby. After restriction mapping and subcloning of the plasmid, the cloned gene was used to detect mRNA levels in several growth conditions. Enzyme activities were measured in various genotypes. At our level of detection ARO7-OSM2 is a low level constitutively expressed gene.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2119-23 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 - PubMed
    1. J Mol Biol. 1976 Aug 15;105(3):427-43 - PubMed
    1. Genetics. 1968 Dec;60(4):735-42 - PubMed
    1. Cell. 1980 Aug;21(1):217-26 - PubMed

LinkOut - more resources