Binding and activity of Bacillus thuringiensis delta-endotoxin to invertebrate cells

Abstract

Fluorescein isothiocyanate was used as a label to detect delta-endotoxin of Bacillus thuringiensis subsp. thuringiensis and israelensis in binding studies with different in vitro cell systems. Protoxin of the subspecies thuringiensis could be labelled directly whereas the activated toxin had to be traced indirectly with labelled antibodies. Both protoxin and activated toxin bound to primary midgut cell cultures of Pieris brassicae larvae as well as to cells of an established culture of Drosophila melanogaster. No binding with either toxin form could be observed with hemocytes of P. brassicae. Biological activity as shown by the trypan blue viability assay was obtained only with the activated toxin against the midgut cells. Toxin of the subspecies israelensis reacted very unspecifically. Binding followed by rapid destruction was obtained with all the tested cultures.