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. 2019 Jan;83(1):181-184.
doi: 10.1080/09168451.2018.1527210. Epub 2018 Oct 5.

Ergothioneine production with Aspergillus oryzae

Shun Takusagawa  1 , Yasuharu Satoh  2 , Iwao Ohtsu  3 , Tohru Dairi  2
Affiliations
Free article

Ergothioneine production with Aspergillus oryzae

Shun Takusagawa et al. Biosci Biotechnol Biochem. 2019 Jan.
Free article

Abstract

To establish a reliable and practical ergothioneine (ERG) supply, we employed fermentative ERG production using Aspergillus oryzae, a fungus used for food production. We heterologously overexpressed the egt-1 and -2 genes of Neurospora crassa in A. oryzae and succeeded in producing ERG (231.0 mg/kg of media, which was 20 times higher than the wild type). Abbreviations: ERG: ergothioneine; HER: hercynine; Cys-HER: hercynylcysteine-sulfoxide; SAM: S-adenosylmethionine; SAH: S-adenosylhomocysteine; l-His: l-histidine; l-Cys: l-cysteine; LC-ESI-MS: liquid chromatography-electrospray ionization-mass spectrometry.

Keywords: Aspergillus oryzae; Ergothioneine; Neurospora crassa; heterologous expression; molecular breeding.

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