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. 2018 Oct 4;10(10):1434.
doi: 10.3390/nu10101434.

Effect of Collagen Hydrolysates from Silver Carp Skin (Hypophthalmichthys molitrix) on Osteoporosis in Chronologically Aged Mice: Increasing Bone Remodeling

Ling Zhang  1 , Siqi Zhang  2 , Hongdong Song  3 , Bo Li  4   5
Affiliations

Effect of Collagen Hydrolysates from Silver Carp Skin (Hypophthalmichthys molitrix) on Osteoporosis in Chronologically Aged Mice: Increasing Bone Remodeling

Ling Zhang et al. Nutrients. .

Abstract

Osteoporosis is a common skeletal disorder in humans and gelatin hydrolysates from mammals have been reported to improve osteoporosis. In this study, 13-month-old mice were used to evaluate the effects of collagen hydrolysates (CHs) from silver carp skin on osteoporosis. No significant differences were observed in mice body weight, spleen or thymus indices after daily intake of antioxidant collagen hydrolysates (ACH; 200 mg/kg body weight (bw) (LACH), 400 mg/kg bw (MACH), 800 mg/kg bw (HACH)), collagenase hydrolyzed collagen hydrolysates (CCH) or proline (400 mg/kg body weight) for eight weeks, respectively. ACH tended to improve bone mineral density, increase bone hydroxyproline content, enhance alkaline phosphatase (ALP) level and reduce tartrate-resistant acid phosphatase 5b (TRAP-5b) activity in serum, with significant differences observed between the MACH and model groups (p < 0.05). ACH exerted a better effect on osteoporosis than CCH at the identical dose, whereas proline had no significant effect on repairing osteoporosis compared to the model group. Western blotting results demonstrated that CHs mainly increased bone remodeling by stimulating the transforming growth factor β1 (TGF-β1)/Smad signaling pathway and improving the interaction between collagen and α2β1 integrin. The results indicated that CHs from fish could be applied to alleviate osteoporosis or treat bone loss.

Keywords: bone remodeling; chronologically aged mice; collagen hydrolysates; osteoporosis.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Effect of collagen hydrolysates on bone mineral density and hydroxyproline content in chronologically aged mice. (A) Bone mineral density; (B) Bone hydroxyproline content. YC, young control group; M, model group; MP, medium dose of proline; LACH, MACH and HACH represent the low, medium and high dose of ACH, respectively; MCCH, medium dose of CCH. * means a significant difference was observed compared to M group (p < 0.05).
Figure 2
Figure 2
Effect of collagen hydrolysates on vertebral histology in chronologically aged mice (HE, 100×). YC, young control group; M, model group; MP, medium dose of proline; LACH, MACH and HACH represent the low, medium and high dose of ACH, respectively; MCCH, medium dose of CCH. Bony trabeculae, bone marrow and cortical bone in lumbar issue were shown as black, blue and green arrows, respectively.
Figure 3
Figure 3
Effect of collagen hydrolysates on the transforming growth factor β (TGF-β)/Smad signaling pathway in chronologically aged mice. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (A) TGF-β; (B) Smad3; (C) Smad7. YC, young control group; M, model group; MP, medium dose of proline; LACH, MACH and HACH represent the low, medium and high dose of ACH, respectively; MCCH, medium dose of CCH. * means a significant difference was observed compared to M group (p < 0.05).
Figure 4
Figure 4
Effect of collagen hydrolysates on the expression of the α2β1 integrin receptor in chronologically aged mice. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (A) α2 subunit; (B) β1 subunit. YC, young control group; M, model group; MP, medium dose of proline; LACH, MACH and HACH represent the low, medium and high dose of ACH, respectively; MCCH, medium dose of CCH. * means a significant difference was observed compared to M group (p < 0.05).
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