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. 2018 Sep 26:11:6259-6269.
doi: 10.2147/OTT.S174524. eCollection 2018.

Effect of long non-coding RNA AOC4P on gastrointestinal stromal tumor cells

Jin-Chen Hu  1 , Quan Wang  2 , Li-Xin Jiang  1 , Li Cai  3 , Hui-Yuan Zhai  1 , Zeng-Wu Yao  1 , Meng-Lai Zhang  1 , Ye Feng  4
Affiliations

Effect of long non-coding RNA AOC4P on gastrointestinal stromal tumor cells

Jin-Chen Hu et al. Onco Targets Ther. .

Retraction in

Abstract

Objective: In this research, we explored the effect of long non-coding RNA (lncRNA) AOC4P on gastrointestinal stromal tumor (GIST) cells.

Materials and methods: The expression of lncRNA AOC4P in tissues was detected by real-time PCR (RT-PCR). The epithelial-mesenchymal transition (EMT)-related proteins in tissues were analyzed by Western blot. The experiment included negative control group (CN), silence AOC4P group (si AOC4P), and silence negative control group (si CT). RT-PCR, MTT, Scratch, Transwell, and Annexin V-FITC methods were used to detect the expression of lncRNA AOC4P, cell proliferation, cell migration ability, cell invasion ability, and apoptosis, respectively. The EMT-related proteins including TGF-β, ZEB1, Vimentin, Snail, and E-cadherin were analyzed by Western blot.

Results: The expression of lncRNA AOC4P and the expression of EMT-related proteins in high-risk GISTs were higher than that in low- and intermediate-risk GISTs (P<0.05). It was revealed that cell proliferative migration and invasive ability in si AOC4P group was decreased than that in CN and si CT groups (P<0.05), and cell apoptosis in si AOC4P group was higher than that in si CT group. The results of Western blot demonstrated that the expression of TGF-β1, ZEB1, Vimentin, and Snail in si AOC4P group were lower than that in si CT and CN group (P<0.05), and the expression of E-cadherin in si AOC4P group was higher than that in si CT and CN group (P<0.05).

Keywords: AOC4P; epithelial-mesenchymal transition; gastrointestinal stromal tumors; long non-coding RNA.

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Conflict of interest statement

Disclosure The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
The relative expression of AOC4P in normal-, high-, and low/medium-risk GIST. Notes: *P<0.05, compared with normal group; #P<0.05, compared with low/medium-risk GIST. Abbreviation: GIST, gastrointestinal stromal tumor.
Figure 2
Figure 2
The EMT-related proteins in tissues. Notes: (A) Protein band, (B) relative expression of TGF-β1, (C) relative expression of ZEB1, (D) relative expression of vimentin, (E) relative expression of snail, and (F) relative expression of E-cadherin. *P<0.05, compared with normal group; #P<0.05, compared with low/medium-risk GIST. Abbreviations: GIST, gastrointestinal stromal tumor; EMT, epithelial–mesenchymal transition.
Figure 2
Figure 2
The EMT-related proteins in tissues. Notes: (A) Protein band, (B) relative expression of TGF-β1, (C) relative expression of ZEB1, (D) relative expression of vimentin, (E) relative expression of snail, and (F) relative expression of E-cadherin. *P<0.05, compared with normal group; #P<0.05, compared with low/medium-risk GIST. Abbreviations: GIST, gastrointestinal stromal tumor; EMT, epithelial–mesenchymal transition.
Figure 3
Figure 3
The proliferative activity of GIST-T1 and GIST-882 cells in CN, si CT, and si AOC4P. Notes: (A) The relative expression of AOC4P was detected by RT-PCR method. (B) The cell viability was measured by MTT method. **P<0.01 indicate statistically significant difference. Abbreviations: GIST, gastrointestinal stromal tumor; CN, negative control group; si CT, silence negative control group; si AOC4P, silence AOC4P group; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide.
Figure 4
Figure 4
The migration ability of GIST-T1 and GIST-882 cells in CN, si CT, and si AOC4P. Notes: (A) The migration ability of GIST-T1 and GIST-882 cells were detected by scratch test. (B) The migration ability of GIST-T1 and GIST-882 cells. **P<0.01 indicate statistically significant difference. Abbreviations: GIST, gastrointestinal stromal tumor; CN, negative control group; si CT, silence negative control group; si AOC4P, silence AOC4P group.
Figure 5
Figure 5
The invasive ability of GIST-T1 and GIST-882 cells in CN, si CT, and si AOC4P. Notes: (A) The invasive ability of GIST-T1 and GIST-882 cells was detected by Transwell method. Magnification ×100. (B) The invasive ability of GIST-T1 and GIST-882 cells. **P<0.01 indicate statistically significant difference. Abbreviations: GIST, gastrointestinal stromal tumor; CN, negative control group; si CT, silence negative control group; si AOC4P, silence AOC4P group.
Figure 6
Figure 6
The apoptosis activity of GIST-T1 and GIST-882 cells in CN, si CT, and si AOC4P. Notes: (A) The apoptosis activity of GIST-T1 and GIST-882 cells. (B) The apoptosis rate of GIST-T1 and GIST-882 cells. **P<0.01 indicate statistically significant difference. Abbreviations: GIST, gastrointestinal stromal tumor; CN, negative control group; si CT, silence negative control group; si AOC4P, silence AOC4P group.
Figure 7
Figure 7
Levels of the related biomarker on EMT in GIST-T1 and GIST-882 cells in CN, si CT, and si AOC4P. Notes: (A) GIST-T1 cells. (B) GIST-882 cells. *P<0.05 indicate statistically significant difference. Abbreviations: GIST, gastrointestinal stromal tumor; CN, negative control group; si CT, silence negative control group; si AOC4P, silence AOC4P group; EMT, epithelial– mesenchymal transition.
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